普洱茶中一株产纤维素酶黑曲霉的分离及鉴定  

Isolation and identification of a cellulase-producing strain Aspergillus niger from Pu‑erh tea

作　　者：欧阳学文 金元宝 朱伟超 吴志华[1,4] 余勃[1,4] 于化泓[1,2] OUYANG Xue-Wen;JIN Yuan-Bao;ZHU Wei-Chao;WU Zhi-Hua;YU Bo;YU Hua-Hong(State Key Laboratory of Food Science and Resources,Nanchang University,Nanchang 330047,China;College of Food Science and Technology,Nanchang University,Nanchang 330047,China;Modern Agriculture and Forestry Engineering College,Ji’an Vocational and Technical College,Ji’an 343000,China;Sino-German Joint Research Institute,Nanchang University,Nanchang 330047,China)

机构地区：[1]南昌大学食品科学与资源挖掘全国重点实验室,南昌330047 [2]南昌大学食品学院,南昌330047 [3]吉安职业技术学院现代农林工程学院,吉安343000 [4]南昌大学中德联合研究院,南昌330047

出　　处：《食品安全质量检测学报》2024年第22期109-115,共7页Journal of Food Safety and Quality

基　　金：国家自然科学基金项目(31860432);江西省自然科学基金项目(20212ACB205013、20212BAB205036);吉安市科技计划项目(20222-181701)。

摘　　要：目的分离、鉴定普洱茶中一株产纤维素酶的黑曲霉(Aspergillus niger)菌株。方法对从普洱茶中得到霉菌菌株进行富集培养和纯化分离,后经过刚果红染色法初筛以及滤纸酶活力法复筛,得到具有较强产纤维素酶能力和较高纤维素酶活力的目的菌株,并测定其酶活力。结果从普洱茶中分离得到24株霉菌菌株,经初步筛选得到7株具有较强产纤维素酶能力的菌株,水解圈直径与菌落直径最大比值达1.76,通过测定滤纸酶活力复筛得到一株具有较高纤维素酶活力的菌株,在发酵第9d时其纤维素酶活力达到16.58U/m L。结合菌落和显微形态鉴定以及菌株ITS基因序列分析确定该菌株为黑曲霉,菌株命名为XO-18。结论本研究筛选获得产纤维素黑曲霉XO-18,该菌株产酶活力高,具有开发成食品工业发酵用菌,满足食品工业需求的潜力。Objective To isolate and identify a cellulase-producing Aspergillus niger strains from Pu-erh tea.Methods Mold strains were isolated and purified from Pu-erh tea after enrichment cultivation,then the target strains were screened by Congo red staining and secondary screening using filter paper enzyme activity method,then obtained the target fungal strains with strong cellulose-degrading ability and high cellulase activity,and measured their enzyme activity.Results Totally 24 mold strains were isolated from Pu-erh tea,and 7 of them showed strong enzyme production ability in preliminary screening.The maximum ratio of hydrolysis ring diameter to colony diameter reached 1.76.The strain with highest cellulase activity was verified to be 16.58 U/mL on the 9th days of fermentation when measured by filter paper assay method of cellulase enzymes.Combined microscopy morphology observation with ITS gene sequence analysis,this strain was identified to be Aspergillus niger,and named as XO-18.Conclusion In this study,the strain of cellulose-producing Aspergillus niger XO-18 is screened and obtained.This strain has high enzyme production activity,and has the potential to be developed into fermentation bacteria for food industry to satisfy the needs of food industry.

关 键 词：黑曲霉 纤维素酶 普洱茶 分离鉴定 

分 类 号：TS201.3[轻工技术与工程—食品科学]