降血脂功能乳酸菌的体外筛选、驯化及鉴定  

In Vitro Screening,Domestication and Identification of Lipid-lowering Functional Lactic Acid Bacteria

作　　者：孙晓琛 张晓千 李玲玉 张瑜[2] 刘小莉 王英[1,2] SUN Xiaochen;ZHANG Xiaoqian;LI Lingyu;ZHANG Yu;LIU Xiaoli;WANG Ying(College of Food and Bioengineering,Jiangsu University,Zhenjiang 212001,China;Institute of Agricultural Processing,Jiangsu Academy of Agricultural Sciences,Nanjing 210014,China)

机构地区：[1]江苏大学食品与生物工程学院,江苏镇江212001 [2]江苏省农业科学院农产品加工研究所,江苏南京210014

出　　处：《食品工业科技》2024年第24期125-132,共8页Science and Technology of Food Industry

基　　金：江苏现代农业产业技术体系建设项目(JATS[2023]441);江苏省科技项目现代农业(BE2022367)。

摘　　要：目的:本研究以来源传统发酵食品中的310株乳酸菌为菌株资源,筛选出具有降血脂功能的乳酸菌。方法:利用邻苯二甲醛法测定菌株胆固醇去除率,采用梯度增加胆固醇浓度的培养方法对初筛菌株进行驯化,单试剂法测定甘油三酯去除率,检测菌悬液及胞内提取物对胆固醇酯酶和胰脂肪酶活性的抑制能力,采用16S rDNA和pheS基因序列对目标菌株进行鉴定。同时对其模拟胃肠道耐受性、自聚集力和疏水性进行研究。结果:初筛菌株BC17经过高浓度胆固醇驯化后,其胆固醇去除率提高到35.70%;该菌株的甘油三酯去除率为58.87%,其胞内提取物对胰脂肪酶和胆固醇酯酶活性的抑制率分别为34.92%和52.95%,菌悬液对两种酶的抑制率分别为7.01%和42.99%;BC17在模拟胃肠道中培养6 h存活率为53.99%;BC17具有较强的自聚集力,24 h可达到69.04%;在乙酸乙酯、二甲苯和氯仿中的疏水率分别为33.71%、38.36%和51.02%;经形态学观察、16S rDNA和pheS基因序列分析,BC17为植物乳植杆菌。结论:植物乳植杆菌BC17具有良好的体外降血脂功能,且具有优良的益生特性,可为降血脂功能发酵产品和益生菌剂的开发提供潜在的菌株资源,应用前景广阔。Objective:In order to screen Lactobacillus species with lipid-lowering function,310 strains of the lactic acid bacteria were obtained from the source of traditional fermented foods.Methods:The o-phthalaldehyde method was applied to measure the strains’rate of cholesterol removal.The culture strategy with a gradient rise in cholesterol concentration was utilized to domesticate the original screening strains.The single-reagent approach was used to determine the rate of triglyceride.The inhibitory potential of bacterial suspensions and intracellular extracts on the activities of cholesterol esterase and pancreatic lipase was tested.The 16S rDNA and the pheS gene sequences were utilized to determine the target bacteria.In addition,the self-aggregation force,hydrophobicity,and simulated gastrointestinal system tolerance were examined.Results:After high concentration cholesterol domestication,the cholesterol removal rate of strain BC17 in the initial screening increased to 35.70%.Additionally,the strain's triglyceride removal rate was 58.87%.Intracellular extracts inhibited the activities of pancreatic lipase and cholesterol esterase by 34.92%and 52.95%,respectively,while its suspensions inhibited the activities of the two enzymes by 7.01%and 42.99%,respectively.After being cultivated for 6 h in a simulated gastrointestinal system,BC17 had a 53.99%survival rate.BC17 had a high self-aggregation rate,which reached 69.04%in 24 h.The hydrophobic rates of ethyl acetate,xylene,and chloroform,respectively,were 33.71%,38.36%,and 51.02%.BC17 was identified as Lactiplantibacillus plantarum based on morphology,16S rDNA and pheS genes sequence analysis.Conclusion:Lactiplantibacillus plantarum BC17 has a good in vitro lipid-lowering activity and outstanding probiotic qualities,thus making it a prospective strain resource for the development of lipid-lowering functional fermentation products and probiotic agents with a wide range of applications.

关 键 词：乳酸菌 降血脂 驯化 胰脂肪酶 胆固醇酯酶 

分 类 号：TS201.3[轻工技术与工程—食品科学]