3种食用菌蛋白质-多糖复合物的体内外降血脂作用  

In Vitro and in Vivo Hypolipidemic Effects of Protein-Polysaccharide Complexes from Three Species of Edible Fungi

作　　者：高琛 李文[2] 陈万超[2] 吴迪[2] 张忠[2] 刘朋 夏永军[1] 杨焱[2] GAO Chen;LI Wen;CHEN Wanchao;WU Di;ZHANG Zhong;LIU Peng;XIA Yongjun;YANG Yan(School of Health Science and Engineering,University of Shanghai for Science and Technology,Shanghai 200093,China;Key Laboratory of Edible Fungi Resources and Utilization(South),Ministry of Agriculture and Rural Affairs,National Engineering Research Center of Edible Fungi,Institute of Edible Fungi,Shanghai Academy of Agricultural Sciences,Shanghai 201403,China)

机构地区：[1]上海理工大学健康科学与工程学院,上海200093 [2]上海市农业科学院食用菌研究所,农业农村部南方食用菌资源利用重点实验室,国家食用菌工程技术研究中心,上海201403

出　　处：《食品科学》2024年第24期117-127,共11页Food Science

基　　金：“十四五”国家重点研发计划重点专项(2023YFF1103401)。

摘　　要：本实验制备了双孢蘑菇、大球盖菇和羊肚菌蛋白质-多糖复合物,并对蛋白质、多糖含量及其氨基酸和单糖组成等进行分析,采用油酸诱导肝细胞株LO-2建立体外高脂血症模型,基于高脂饮食建立高脂血症的小鼠模型,探究双孢蘑菇、大球盖菇和羊肚菌蛋白质-多糖复合物的体内外降血脂作用。结果表明,双孢蘑菇、大球盖菇和羊肚菌蛋白质-多糖复合物的蛋白质相对含量分别为(55.56±0.51)%、(52.41±0.31)%、(54.96±0.47)%;多糖相对含量分别为(21.98±1.17)%、(31.95±0.52)%、(15.55±0.78)%。体外细胞实验结果显示,3种食用菌蛋白质-多糖复合物可显著抑制肝细胞中的脂滴聚集;动物实验结果表明,双孢蘑菇、大球盖菇和羊肚菌蛋白质-多糖复合物均能明显抑制高脂饮食小鼠的体质量增长、甘油三酯和低密度脂蛋白胆固醇水平升高、脂肪组织细胞的肿大以及肝脏组织中脂质沉积。高剂量组的双孢蘑菇、大球盖菇和羊肚菌蛋白质-多糖复合物可将高脂血症小鼠肝脏中脂质相对含量从(30.59±0.38)%分别降至(13.74±1.57)%、(9.68±0.73)%、(7.15±0.39)%,对肝脏脂滴形成的抑制程度可分别达到(55.09±5.12)%、(68.34±2.37)%、(76.64±1.28)%。此外,双孢蘑菇、大球盖菇和羊肚菌蛋白质-多糖复合物对长期高脂饮食引起的肝功能损伤具有良好的改善作用。综上,双孢蘑菇、大球盖菇和羊肚菌蛋白质-多糖复合物具有调节脂质代谢、减轻肝损伤程度、缓解高血脂症的作用,本研究可为食用菌相关功能产品的开发提供参考。Protein-polysaccharide complexes of Agaricus bisporus,Stropharia rugosa and Morchella esculenta were prepared and their protein and polysaccharide contents as well as amino acid and monosaccharide composition were evaluated.The in vitro and in vivo lipid-lowering effects of the complexes on oleic acid-induced liver LO-2 cells and high-fat diet fed mice were investigated.The results showed that the protein contents of the three complexes were(55.56±0.51)%,(52.41±0.31)%,and(54.96±0.47)%,respectively,and the polysaccharide contents were(21.98±1.17)%,(31.95±0.52)%,and(15.55±0.78)%,respectively.In vitro cell experiments showed that all the complexes significantly inhibited the accumulation of lipid droplets in hepatocytes.Animal experiments showed that they significantly inhibited the increase in body mass,triglyceride(TG)and low-density lipoprotein cholesterol(LDL-C)levels,blocked the swelling of the swelling of hepatocytes,and inhibited hepatic lipid deposition in mice fed a high-fat diet.In the high-dose group,the hepatic lipid content of mice with hyperlipidemia was reduced from (30.59 ± 0.38)% to (13.74 ± 1.57)%, (9.68 ± 0.73)%, and (7.15 ±0.39)%, respectively, by the complexes from A. bisporus, S. rugosa and M. esculenta, and the inhibition rates of hepaticlipid droplets by these complexes were (55.09 ± 5.12)%, (68.34 ± 2.37)% and (76.64 ± 1.28)%, respectively. In addition, theprotein-polysaccharide complexes alleviated liver function damage caused by long-term high-fat diet. In summary, it canbe concluded that A. bisporus, S. rugosa and M. esculenta protein-polysaccharide complexes can regulate lipid metabolism,alleviate liver injury and hyperlipidemia, which provides a reference for the development of functional products based onedible fungi.

关 键 词：食用菌 蛋白质 多糖 降血脂 

分 类 号：TS201.4[轻工技术与工程—食品科学]