基于复合凝聚法制备红曲色素微胶囊及其稳定性表征  

Preparation and Stability of Monascus Pigment Microcapsules by Complex Coacervation

作　　者：陈师昀 宋畅 陈艺玲 侯思文 梁梓华 李文龙 吕旭聪 倪莉[1,2] CHEN Shiyun;SONG Chang;CHEN Yiling;HOU Siwen;LIANG Zihua;LI Wenlong;LÜXucong;NI Li(Institute of Food Science and Technology,College of Biological Science and Engineering,Fuzhou University,Fuzhou 350108,China;Food Nutrition and Health Research Center,School of Advanced Manufacturing,Fuzhou University,Jinjiang 362200,China)

机构地区：[1]福州大学生物科学与工程学院,食品科学技术研究所,福建福州350108 [2]福州大学先进制造学院,食品营养与健康研究中心,福建晋江362200

出　　处：《食品科学》2024年第23期221-231,共11页Food Science

基　　金：中央引导地方科技发展资金专项(2022L3075);福建省自然科学基金面上项目(2022J01101);福州大学“旗山学者”高层次人才计划项目(GXRC21049)。

摘　　要：为提高红曲色素的稳定性,采用明胶和阿拉伯胶作为壁材,辅之以谷氨酰胺转氨酶和羟丙基-β-环糊精,通过复合凝聚法制备红曲色素微胶囊。利用Zeta电位、浊度测定确定复合凝聚的工艺条件为明胶与阿拉伯胶的最佳比例为1∶1、反应最适pH值为4.0。在此基础上,以红曲色素包埋效率为响应值,通过响应面法优化微胶囊制备工艺条件为壁材添加量1.5%、芯壁比2.89∶1、凝聚时间42 min,在此条件下红曲色素的包埋效率为85.06%。对红曲色素微胶囊的溶解性、贮藏稳定性、光稳定性及体外模拟胃肠道释放特性进行表征,结果显示:微胶囊显著提高了红曲色素的水溶性和贮藏稳定性,且在低温及室温条件下贮藏最为稳定;通过模拟红曲色素及其微胶囊的光降解过程,发现红曲红色素和橙色素符合一级动力学模型,黄色素符合零级动力学模型,微胶囊能显著提高红曲色素的光稳定性;微胶囊体系对延缓红曲色素在模拟胃液中的释放有良好的效果,在模拟肠液中的释放速度明显高于模拟胃液,这表明微胶囊体系可以很好地控制红曲色素在模拟胃肠液中的释放。In order to improve the stability of Monascus pigments(MPs),gelatin and Arabic gum were used as wall materials,supplemented with transglutaminase(TGase)and hydroxypropyl-β-cyclodextrin,to prepare MP microcapsules by composite coagulation.Based on zeta potential and turbidity,the optimal gelatin-Arabic gum ratio and pH were determined to be 1:1 and 4.0,respectively.Furthermore,using response surface methodology,the optimal process conditions that provide maximum microencapsulation efficiency(85.06%)were established as follows:wall material concentration of 1.5%,coreto-wall ratio of 2.89:1,and coacervation time of 42 min.The solubility,storage stability,photostability and in vitro release characteristics of the microcapsules were characterized.The results showed that the microcapsules significantly improved the water solubility and storage stability of MPs and were the most stable under low temperature and room temperature conditions.The photodegradation processes of free and microencapsulated Monascus red and orange pigments followed the first-order kinetic model,while the photodegradation process of free and microencapsulated Monascus yellow pigments obeyed the zero-level kinetic model.Microencapsulation significantly improved the photostability of MPs.In addition,the microcapsules had good slow-release performance in simulated gastric fluid,and the release rate was significantly lower than in simulated intestinal fluid,indicating that the microcapsule system could well control the release of MPs in simulated gastrointestinal fluid.

关 键 词：红曲色素 复合凝聚法 微胶囊 稳定性 

分 类 号：TS201[轻工技术与工程—食品科学]