低能N^+离子注入谷氨酸产生菌诱变选育及其发酵的研究  

Study of the Mutation Breeding and Fermentation on Glutamic Acid Yielding Bacterium with Low Energy N^+ Ion Beam Implantation

作　　者：甄卫军[1] 李茜[2] 张石峰[2] 曾宪贤[2] 高林[1] 李江[3] 

机构地区：[1]中国科学院新疆化学所 [2]新疆大学离子束生物工程中心,乌鲁木齐830008 [3]新疆石河子味精厂,石河子832000

出　　处：《中国科学院研究生院学报》2002年第4期428-432,共5页Journal of the Graduate School of the Chinese Academy of Sciences

基　　金：新疆维吾尔自治区自然科学基金项目(2 0 0 0 1);乌鲁木齐重大科技攻关项目(Y0 0 10 )资助

摘　　要：采用低能N+离子注入技术,以D1 1 0 和D1 1 0B为出发菌进行了诱变选育高产谷氨酸菌株和发酵的试验研究。通过研究已经初筛到两株高产菌株D52 2 1 和B32 63,其多批次摇瓶平均产酸分别达到 8.4%、7.5 %,比出发菌分别提高 3 5.48%和 2 5 %.其一级种子生长曲线比原出发菌有明显变化,高产菌株发酵对数期平均提前 2～ 3h.从发酵曲线显示出高产菌株的代谢活力增强,倍增时间缩短。Low energy N + ion implantation technology is used in the mutation breeding and fermentation on glutamic acid yielding starting strains of D 110 and D 110B . It is showed in this study that two high yielding glutamic acid strains D 5221 and B 3263 are screened out and the average rocker bottle fermentation GA(%) can achieve 8.4%、7.5%, they are respectively raised by 35.48%、25% compared to the original strains .There are clearly improved in the aspect on the first class seed fermentation curve. And their logarithm phase has generally improved by 2～3 h. From the fermentation curve it is showed that their metabolic capacity are improved, the multiplication cycle are shortened.So it will play a positive role in reducing the fermentation cycle.

关 键 词：低能N^+离子注入 谷氨酸 诱变选育 发酵生产 味精 发酵周期 

分 类 号：TS264.23[轻工技术与工程—发酵工程] TS2[轻工技术与工程—食品科学与工程]