超高效液相色谱-质谱法测定软糖中赤藓糖醇、木糖醇、山梨糖醇和麦芽糖醇  

Determination of Erythritol,Xylitol,Sorbitol,and Maltitol in Gummies by Ultrahigh Performance Liquid Chromatography-Mass Spectrometry Method

作　　者：侯少平 胡杰 HOU Shaoping;HU Jie(Access(Guangzhou)Testing Technology Service Co.Ltd.,Guangzhou 510730,Guangdong,China)

机构地区：[1]捷通(广州)检测技术服务有限公司,广东广州510730

出　　处：《甘蔗糖业》2024年第6期83-93,共11页Sugarcane and Canesugar

摘　　要：建立一种超高效液相色谱-质谱(UPLC-QDa)的检测方法,用于检测软糖中赤藓糖醇、木糖醇、山梨糖醇和麦芽糖醇含量。样品经70℃水超声提取30 min,用70%乙腈-水(V/V)稀释后,经超高效液相色谱-质谱分析。流动相为乙腈-含0.1%的氨水溶液,梯度洗脱,流速为0.2 mL/min,柱温为40℃,样品进样量为1μL,以保留时间选择离子定性,以外标法定量。赤藓糖醇、木糖醇、山梨糖醇和麦芽糖醇在5~160μg/mL浓度范围之间具有良好的线性关系(R2>0.99);加标回收率在90.44%~104.46%之间;方法精密度相对标准偏差RSD(n=6)在1.84%~4.19%之间。本方法可应用于软糖中赤藓糖醇、木糖醇、山梨糖醇和麦芽糖醇的测定。Establish an ultra-high performance liquid chromatography-mass spectrometry detection method for detecting erythritol,xylitol,sorbitol,and maltitol content in gummies.The sample was extracted by water ultrasound at 70℃for 30 min,diluted with 70%acetonitrile-water(V/V),and analyzed by ultra-high performance liquid chromatography-mass spectrometry(UPLC-QDa).The mobile phase is acetonitrile-0.1%ammonia aqueous solution,gradient elution at a flow rate of 0.2 mL/min and column temperature at 40℃,and the sample injection volume is 1μL.Sugar alcohol was qualitatively determined by retention time and selected ions and quantified by external standard method.The linear relationship of erythritol,xylitol,sorbitol,and maltitol is good(R2>0.99)in the concentration range of 5~160μg/mL.The spiked recoveries are within the range of 90.44%~104.46%.The relative standard deviation(RSD)of method precision is 1.84%~4.19%(n=6).This method is applicable for the determination of erythritol,xylitol,sorbitol,and maltitol in gummies.

关 键 词：超高效液相色谱-质谱联用法 软糖 赤藓糖醇 木糖醇 山梨糖醇 麦芽糖醇 

分 类 号：TS245[轻工技术与工程—制糖工程] O657.63[轻工技术与工程—食品科学与工程]