植物蛋白基玉米黄色素微胶囊的制备及性能评价  

Preparation and Performance Evaluation of Plant Protein-Based Maize Yellow Pigment Microcapsules

作　　者：高莉[1] 杨俊燕 赵英虎[1] 季海霞 陈佳慧 郝媛 石英 郝瑞 GAO Li;YANG Junyan;ZHAO Yinghu;JI Haixia;CHEN Jiahui;HAO Yuan;SHI Ying;HAO Rui(School of Chemistry and Chemical Engineering,North University of China,Taiyuan 030051,China)

机构地区：[1]中北大学化学与化工学院,山西太原030051

出　　处：《食品科学》2025年第5期65-74,共10页Food Science

基　　金：山西省重点研发计划项目(202302140601014);山西省回国留学人员科研资助项目(2020-118);医药生物技术山西省重点实验室开放课题基金项目(KF202002);山西省研究生教育教学改革课题(2022YJJG185);山西省研究生创新项目(2022Y616,2023KY604,2023SJ203);山西省自然科学基金项目(202303021222093);山西省高等教育机构科技创新项目(2310400081MZ)。

摘　　要：为了改善玉米黄色素(maize yellow pigment,MYP)水溶性差、不稳定性和生物利用度低等问题,本实验以不同植物基蛋白(豌豆分离蛋白、火麻蛋白、大豆分离蛋白)和海藻酸钠两类材料结合作为复合壁材,以麦芽糊精为单一壁材分别制备MYP微胶囊,对微胶囊的包埋效果、理化性质、粒径、热稳定性、微观结构、体外消化特性以及抗氧化能力进行评价,并研究其对H_(2)O_(2)诱导的ARPE-19细胞氧化损伤模型的保护作用。结果表明,MYP的微胶囊化可以提高其溶解度、生物利用度和抗氧化活性;微胶囊(MYP质量浓度在375~1 125μg/mL范围内)不影响ARPE-19细胞活力,并且在H_(2)O_(2)诱发氧化损伤模型中显示出更好的细胞抗氧化作用;3种壁材展现出不同的功能特性。与其他壁材相比,豌豆蛋白-海藻酸钠壁材的微胶囊有较高的包埋率、较低的含水量、较小尺寸,同时具有良好的流动性和稳定性。To overcome the problems associated with maize yellow pigment(MYP)such as poor water solubility,instability,and low bioavailability,MYP was microencapsulated with different plant-based proteins,pea protein isolate(PPI),hemp protein(HP),or soybean protein isolate(SPI)combined with sodium alginate(SA)as a mixed wall materials or maltodextrin as a single wall material.The encapsulation efficiency,physicochemical properties,particle size,thermal stability,microstructure,in vitro digestion characteristics,and antioxidant capacity of the microcapsules were evaluated,and their protective effect on H_(2)O_(2)-induced oxidative damage in ARPE-19 cells was investigated.The results showed that microencapsulation of MYP improved its solubility,bioavailability and antioxidant activity.Microcapsules with MYP concentrations in the range of 375–1125μg/mL did not affect the viability of ARPE-19 cells,and showed superior cellular antioxidant effect in the H_(2)O_(2)-induced oxidative damage model.The three mixed wall materials exhibited different functional characteristics.Compared with those prepared with the other wall materials,PPI-SA microcapsules showed higher encapsulation efficiency,lower water content,smaller particle size,and better fluidity and stability.

关 键 词：玉米黄色素 植物蛋白基 复凝聚法 壁材 微胶囊 

分 类 号：TS209[轻工技术与工程—食品科学]