三地羊肚菌菌丝体胞外多糖分离纯化及降血糖和抗氧化活性研究  

Extraction and isolation of Morchella eohespera mycelium extracellular polysaccharides and its hypoglycemic and antioxidant activities

作　　者：刘冰[1] 李沛 曾馨俞 刘小春 邹名洋 林杉 赵萍[1] 曹建雄 LIU Bing;LI Pei;ZENG Xin-Yu;LIU Xiao-Chun;ZOU Ming-Yang;LIN Shan;ZHAO Ping;CAO Jian-Xiong(School of Life Science and Engineering,Lanzhou University of Technology,Lanzhou 730050,China;School of Science,Lanzhou University of Technology,Lanzhou 730050,China)

机构地区：[1]兰州理工大学生命科学与工程学院,兰州730050 [2]兰州理工大学理学院,兰州730050

出　　处：《食品安全质量检测学报》2025年第5期86-93,共8页Journal of Food Safety and Quality

基　　金：甘肃省知识产权计划项目(22ZSCQ005,22ZSCQ008);兰州理工大学博士科研启动基金项目(062002)。

摘　　要：目的 以三地羊肚菌菌丝体胞外多糖(Morchella eohespera mycelium extracellular polysaccharides, MEP)为研究对象,采用DEAE琼脂糖凝胶FF(DEAE Sepharose Fast Flow)柱层析纯化得到多糖MEP-H和MEP-N,分别对其理化性质、体外降血糖活性和抗氧化活性进行分析。方法 通过碳水化合物含量测定、紫外扫描分析、傅里叶变换红外光谱分析和扫描电子显微镜对多糖MEP-H和MEP-N进行理化性质研究;通过α-淀粉酶抑制率、α-葡萄糖苷酶抑制率、1,1-二苯基-2-三硝基苯肼(1,1-diphenyl-2-picrylhydrazyl, DPPH)自由基清除能力、2,2’-联氮-二(3-乙基-苯并噻唑啉-6-磺酸)二铵盐[2,2’-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid)ammonium salt,ABTS]阳离子自由基清除能力、还原力和超氧阴离子清除能力,评价MEP-H和MEP-N的体外降血糖活性和抗氧化活性。结果 三地羊肚菌菌丝体胞外多糖MEP-H和MEP-N碳水化合物含量分别为(78.12±0.14)%和(77.37±0.03)%。降血糖研究表明,当质量浓度0.75 mg/mL时, MEP-H和MEP-N对α-淀粉酶抑制率最高,分别为(8.06±1.93%)和(11.08±1.05)%;质量浓度0.50mg/m L时,MEP-H和MEP-N对α-葡萄糖苷酶抑制率最高,分别为(74.93±2.72)%和(69.48±2.97)%。抗氧化研究表明, MEP-H和MEP-N在质量浓度分别为4 mg/mL、2 mg/mL时达到最佳DPPH自由基清除活性,清除率分别为(47.54±10.88)%和(47.16±6.91)%;质量浓度为8mg/mL时,MEP-H和MEP-N的最大ABTS阳离子自由基清除率分别为(8.67±0.53)%和(17.00±4.21)%,最大还原力吸光值分别为0.13±0.004和0.17±0.008,最大超氧阴离子清除率分别为(40.95±6.02)%和(29.87±3.18)%。结论 羊肚菌菌丝体胞外多糖MEP-H和MEP-N均具有降血糖活性和抗氧化活性,本研究为液态发酵羊肚菌的深入研究提供了理论依据。Objective To study the Morchella eohespera mycelium extracellular polysaccharides(MEP),purify MEP-H and MEP-N by DEAE Sepharose Fast Flow column chromatography,and analyze their physicochemical properties,hypoglycemic activities,and antioxidant activities in vitro.Methods The physicochemical properties of MEP-H and MEP-N were studied by carbohydrate content determination,analysis of ultraviolet scanning,Fourier transform infrared spectroscopy analysis,and scanning electron microscopy.The hypoglycemic activities and antioxidant activities of MEP-H and MEP-N were evaluated byα-amylase inhibition rate,α-glucosidase inhibition rate,1,1-diphenyl-2-picrylhydrazyl(DPPH)free radical scavenging ability,2,2’-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid)ammonium salt(ABTS)cationic free radical scavenging ability,reducing power and superoxide anion scavenging ability.Results The carbohydrate content of MEP-H and MEP-N was(78.12±0.14)%and(77.37±0.03)%,respectively.Hypoglycemic studies showed that at a mass concentration of 0.75 mg/mL,MEP-H and MEP-N had the highestα-amylase inhibition rates,which were(8.06±1.93)%and(11.08±1.05)%,respectively;at a mass concentration of 0.50 mg/mL,MEP-H and MEP-N had the highestα-glucosidase inhibition rates,which were(74.93±2.72)%and(69.48±2.97)%,respectively.Antioxidant studies showed that MEP-H and MEP-N achieved the best DPPH free radical scavenging activity at mass concentrations of 4 mg/mL and 2 mg/mL,with scavenging rates of(47.54±10.88)%and(47.16±6.91)%,respectively;at a mass concentration of 8 mg/mL,the maximum ABTS cationic free radical scavenging rates of MEP-H and MEP-N were(8.67±0.53)%and(17.00±4.21)%,respectively,the maximum reducing power absorbance values were 0.13±0.004 and 0.17±0.008 respectively,and the maximum superoxide anion scavenging rates were(40.95±6.02)%and(29.87±3.18)%,respectively.Conclusion Both MEP-H and MEP-N,the extracellular polysaccharides from the mycelium of Morchella eohespera,exhibit hypoglycemic and antioxidant activities.This stud

关 键 词：三地羊肚菌菌丝体 胞外多糖 理化性质 降血糖活性 抗氧化活性 

分 类 号：TS201.2[轻工技术与工程—食品科学]