酿酒酵母YY-02产胞外多糖发酵条件优化及其功能特性分析  

Optimization of fermentation conditions of exopolysaccharide production from Saccharomyces cerevisiae YY-02 and its functional properties analysis

作　　者：王长丽 张称 宾晓芸[1] 赵云慧 张云野 叶广彬 WANG Changli;ZHANG Cheng;BIN Xiaoyun;ZHAO Yunhui;ZHANG Yunye;YE Guangbin(School of Basic Medical Sciences,Youjiang Medical University for Nationalities,Baise 533000,China;School of Laboratory Medicine,Youjiang Medical University for Nationalities,Baise 533000,China;School of Medical Imaging,Youjiang Medical University for Nationalities,Baise 533000,China;School of General Medicine,Youjiang Medical University for Nationalities,Baise 533000,China;Institute of Life Sciences,Youjiang Medical University for Nationalities,Baise 533000,China)

机构地区：[1]右江民族医学院基础医学院,广西百色533000 [2]右江民族医学院医学检验学院,广西百色533000 [3]右江民族医学院医学影像学院,广西百色533000 [4]右江民族医学院全科医学院,广西百色533000 [5]右江民族医学院生命科学研究院,广西百色533000

出　　处：《中国酿造》2025年第3期214-222,共9页China Brewing

基　　金：广西高校中青年教师基础能力提升项目(2022KY0547);国家级大学生创新创业训练计划项目(202210599020);自治区教育厅创新创业训练计划项目(S202210599042,S202210599065,S202210599091);2021年右江民族医学院“新启航”青年骨干项目(DC2200001896)。

摘　　要：该研究以酿酒酵母(Saccharomyces cerevisiae)YY-02为供试菌株,以初始发酵培养基为培养基质发酵产胞外多糖(EPS),采用单因素试验及响应面法优化其发酵条件,并对其EPS的功能特性(持水性、水溶性、乳化性及抗氧化特性)进行研究。结果表明,菌株YY-02产胞外多糖最佳培养基配方为:蔗糖73 g/L、酵母提取物21 g/L、NH4Cl 2.0 g/L,KH2PO42.0 g/L、CaCl20.3 g/L;最佳培养条件为装液量141 mL/250 mL、接种量2%、初始pH 7.0、发酵时间144 h及发酵温度30℃。在此优化条件下,EPS产量达到4.52 g/L,是优化前的2.50倍。菌株YY-02所产EPS的总糖、糖醛酸、蛋白质、硫酸盐含量分别为83.65%、15.27%、0.78%和0.30%,其持水性(WHC)和水溶性指数(WSI)分别为229.67%、75.23%;当EPS质量浓度为1 g/L时,对有机相汽油、大豆油、苯甲醛、石油醚的乳化性均>25%;当EPS质量浓度为3 mg/mL,对2,2'-联氮-双-3-乙基苯并噻唑啉-6-磺酸(ABTS+)、O2-自由基及H2O2的清除率最高,分别为24.8%、43.79%和11.40%。因此,酿酒酵母YY-02具有一定的抗氧化活性。Exopolysaccharide(EPS)was produced by fermentation using Saccharomyces cerevisiae YY-02 as the test strain and initial fermentation medium as the culture medium.The fermentation conditions were optimized by single factor tests and response surface tests,and the functional properties(water retention,water solubility,emulsification and antioxidant properties)of EPS were studied.The results showed that the optimal medium formula for polysaccharide production by strain YY-02 was as follows:sucrose 73 g/L,yeast extract 21 g/L,NH4Cl 2.0 g/L,KH2PO42.0 g/L,CaCl20.3 g/L.The optimal culture conditions were obtained as follows:loading liquid volume 141 ml/250 ml,inoculum 2%,initial pH 7.0,fermentation time 144 h,and temperature 30℃.Under this optimization conditions,EPS yield reached 4.52 g/L,which was 2.50 times more than before optimization.The contents of total sugar,uronic acid,protein and sulfate of EPS produced by strain YY-02 were 83.65%,15.27%,0.78%and 0.30%,respectively.The water retention(WHC)and water solubility index(WSI)were 229.67%and 75.23%,respectively.When the mass concentration of EPS was 1 g/L,the emulsification of organic phase gasoline,soybean oil,benzaldehyde and petroleum ether was more than 25%.When the mass concentration of EPS was 3 g/L,the scavenging rates of 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid)(ABTS+),O2-free radical and H2O2 were the highest,which were 24.8%,43.79%and 11.40%,respectively.Therefore,S.cerevisiae YY-02 had certain antioxidant activity.

关 键 词：酿酒酵母 胞外多糖 功能特性 发酵条件优化 响应面法 

分 类 号：TS201.3[轻工技术与工程—食品科学]