蔗汁中蔗糖含量的分光光度法测定  

Spectrophotometric Method for Determination of Sucrose Content in Sugarcane Juice

作　　者：李利军[1] 孔红星[1] 何云[1] 赖学寨[1] 张家仁[1] 

机构地区：[1]广西工学院轻工化工系,广西柳州545006

出　　处：《分析测试学报》2003年第4期51-54,共4页Journal of Instrumental Analysis

基　　金：广西自然科学基金资助项目 (0146001) ;广西教育厅科研基金资助项目 (桂教科研[2000]392)

摘　　要：建立了测定蔗糖的分光光度法 ,在酸性介质中 ,蔗糖还原磷钼黄生成磷钼蓝 ,对该反应进行了详细的研究 ,确定了最佳反应条件 ;结果表明 ,所生成的磷钼蓝最大吸收波长在820nm ,蔗糖溶液在0.57～22.56mg/L服从比尔定律 ,表观摩尔吸光系数为1.17×104 L/(mol·cm) ,定量下限为0.57mg/L;该法灵敏度高、选择性好 ,用于甘蔗汁样品的测定 ,结果令人满意 ;A method for determination of sucrose was developed by spectrophotometry.It was found that in sulˉphuric acid medium sucrose can reduce phosphorus molybdenum yellowto form phosphorus molybdenum blue.The reaction was investigated in detail and the optimal reaction conditions were obtained.The maximum absorption wavelength of resulting phosphorus molybdenum blue is at820nm.Beer's law is obeyed over the concentration range of0.57～22.56mg/L of sucrose and the apparent molar absorbancy index is1.17×10 4 L/(mol·cm).The method was applied to the determination of sucrose content in sugarcane juice samples with satisfactory results.The mechanism of reaction of sucrose with phosphorus molybdenum yellowwas discussed.

关 键 词：蔗汁 蔗糖 含量 分光光度法 测定 磷钼蓝 磷钼黄 

分 类 号：TS242.1[轻工技术与工程—制糖工程] O657.3[轻工技术与工程—食品科学与工程]