高浓度双乙酰定向驯化获得优良啤酒酵母菌株的研究  

作　　者：张秀丽[1] 唐晓达[1] 刘进文[1] 刘月英[1] 魏文铃[1] 戴玉聪 林英辉 

机构地区：[1]厦门大学生命科学学院,厦门361005 [2]福建雪津啤酒集团公司,莆田351111

出　　处：《食品工业科技》2003年第8期42-43,共2页Science and Technology of Food Industry

摘　　要：在双乙酰浓度为1mg/mL的12°Bx麦芽汁中进行高浓度定向驯化,经涂布抗双乙酰固体选择培养基,从啤酒酿造生产菌株啤酒酵母(Saccharomycessp.)A中富集筛选分离得到一株双乙酰还原速度优于亲株A的新菌株TA4。以12°Bx麦芽汁为培养基用内装300mL麦芽汁的500mL三角瓶10℃下发酵,发酵8d后发酵液双乙酰含量比亲株降低了36.31%,发酵度提高4.5%,且该菌株的絮凝性、发酵速率等特性仍保持了亲株A的优良性状。After domestication with diacetyl (1mg /mL)in25mL 12°Bx wort,a Saccharomyces sp.strain TA4was obtained from orig inal strain A,a strain for beer production.The diacetyl content in the fermented liquid during the fermentation was investig ated in500mL flask with300mL 12°Bx wort at 10℃.After 8days,the diacetyl content in the fermentation broth of TA4was 0.100mg /L,36.31%lower than that of its orig inal strain A(0.157mg /L).The true fermentation deg ree of strain TA4was 4.5%hig her than that of its orig inal strain.Some physical properties were measured.The results showed that flocculation and fermentation rate of strain TA4were the same to the orig inal strain A

关 键 词：双乙酰 定向驯化 啤酒酵母菌株 生理性状 菌种选育 

分 类 号：TS261.11[轻工技术与工程—发酵工程] TS262.5[轻工技术与工程—食品科学与工程]