响应面法优化黄粉虫蛋白制备ACE抑制肽的条件  

Optimization of Preparation of Angiotensin-I Converting Enzyme Inhibitory Peptides Derived from Terebrio molitor L. Protein by Response Surface Methodology

作　　者：崔楠[1] 陶晓赟[1] 李娟[1] 陈健[1] 赵立仪 孙爱东[1] 

机构地区：[1]北京林业大学生物科学与技术学院 林业食品加工与安全北京市重点实验室,北京100083

出　　处：《食品科学》2014年第15期156-160,共5页Food Science

基　　金：国家高技术研究发展计划(863计划)项目(2013AA102206)

摘　　要：以黄粉虫蛋白粉为原料,利用酶解技术对制备血管紧张素转换酶(angiotensin converting enzyme,ACE)抑制肽进行优化。通过单因素及响应面试验,确定木瓜蛋白酶的酶解工艺,利用酶标法测定酶解产物的ACE抑制率,研究底物质量浓度、加酶量、pH值、酶解时间、酶解温度对ACE抑制肽活性的影响。结果表明:当底物质量浓度为7 g/100 mL、加酶量1%、pH 6.5、酶解时间7 h、酶解温度55℃时,黄粉虫蛋白粉酶解产物的ACE抑制率达到58.86%。In this study, Tenebrio molitor protein powder was hydrolyzed by papain to prepare angiotensin-I converting enzyme(ACE) inhibitory peptides. Single factor design(involving temperature, pH, substrate concentration, enzyme dosage and hydrolysis time) and response surface methodology were used to determine the optimal hydrolysis conditions for preparing ACE inhibitory peptides. ACE inhibitory rates of hydrolysates were determined by enzyme-linked immunosorbent assay(ELISA). The optimum conditions were determined as follows: substrate concentration 7 g/100 mL, enzyme dosage 1%, pH 6.5, hydrolysis time 7 h and temperature 55 ℃. The ACE inhibitory rate obtained under these conditions was 58.86%.

关 键 词：黄粉虫蛋白粉 木瓜蛋白酶 ACE抑制肽 酶解条件 

分 类 号：TS218[轻工技术与工程—粮食、油脂及植物蛋白工程]