响应面优化罗耳阿太菌胞外多糖提取工艺及其保湿、黏度特性分析  

Extraction and Viscosity Stability of Exopolysaccharides with Moisture-Retention Capacity from Athelia rolfsii

作　　者：魏明[1] 李鸿梅[1] 马艳秋[2] 赵慧[1] 张桂弘[1] 闵伟红[1] 

机构地区：[1]吉林农业大学食品科学与工程学院,吉林长春130118 [2]吉林农业大学图书馆,吉林长春130118

出　　处：《食品科学》2015年第8期67-73,共7页Food Science

基　　金：吉林省科技发展计划项目(20126037)

摘　　要：利用响应面分析法优化乙醇醇沉提取罗耳阿太菌胞外多糖(Athelia rolfsii exopolysaccharides,AEPS)工艺,并探究其保湿特性及黏度稳定性。以AEPS提取量为指标,在单因素试验的基础上,应用Box-Behnken试验设计优化多糖提取工艺。以壳聚糖和尿素作对照,研究AEPS吸湿及保湿性能,同时研究温度、pH值和离子(Mg2+、Ca2+、Na+、K+)浓度对AEPS黏度稳定性的影响。结果表明:AEPS醇沉最佳条件为浓缩倍数3、醇沉时间17 h、醇沉温度5℃、无水乙醇体积为发酵液的1.7倍,所得AEPS提取量为12.24 g/L;AEPS吸湿及保湿性能明显优于壳聚糖和尿素,1 mg/m L AEPS(黏度57 m Pa·s)保湿性最好;在5~95℃范围内,35℃时AEPS黏度最低,35℃以外多糖黏度都高于51 m Pa·s,但在pH 1~12、离子浓度0~1.0 mol/L范围内,AEPS黏度几乎不受影响。The conditions of ethanol precipitation for Athelia rolfsii exopolysaccharides(AEPS) were optimized by response surface methodology. Moisture-retention capacity and viscosity stability of AEPS were also determined. Using AEPS yield as the response variable, optimal ethanol precipitation process for AEPS was determined by Box-Behnken design based on single factor tests. Compared to chitosan and urea, the moisture-absorption and moisture-retention capacities of AEPS were also studied. In addition, the effects of temperature, pH and ion concentration(Mg2+, Ca2+, Na+, and K+) on the viscosity stability of AEPS were examined. The optimal conditions of ethanol precipitation for AEPS were determined as follows: 3-fold condensation of the fermentation supernatant before 17 h precipitation at 5 ℃ with an ethanol/fermentation supernatant ratio of 1.7:1(V/V). Under these conditions, the AEPS yield was 12.24 g/L. The moisture-absorption and moisture-retention capacities of AEPS were superior to those of chitosan and urea. The optimal concentration for moisture-retention was 1 mg/m L(57 m Pa·s). At temperatures in the range of 5–95 ℃, the lowest viscosity of AEPS was 51 m Pa·s at 35 ℃. However, AEPS viscosity was very stable in the pH range of 1 to 12 and at ion concentrations ranging from 0 to 1.0 mol/L.

关 键 词：罗耳阿太菌胞外多糖 醇沉 吸湿性 保湿性 黏度稳定性 

分 类 号：TS201.2[轻工技术与工程—食品科学]