絮凝性强的优良面包酵母菌株的选育  

Breeding of Excellent Baker's Yeast Strain with Good Flocculation

作　　者：刘春秀[1] 何秀萍[1] 蒋思欣[1] 曲娜[1] 张博润[1] 

机构地区：[1]中国科学院微生物研究所,北京100080

出　　处：《微生物学报》2003年第5期659-665,共7页Acta Microbiologica Sinica

摘　　要：通过初筛、单倍体分离、DES诱变、絮凝基因的克隆表达及杂交等育种技术成功构建了高生物量、耐高糖、强絮凝的优良面包酵母菌株 (Saccharomycescerevisiae)ZLTH 5 8(MATa α,leu ,FLO1 )。菌株ZLTH 5 8具有双亲的优良性状 ,遗传性状稳定。对其生物量、耐高糖能力、絮凝特性进行了检测 ,结果表明 ,菌株ZLTH 5 8的生物量是原始亲株BL5 6的 1 2 1倍 ;耐高糖能力优于原始亲株BL61 ;絮凝性能明显优于原始亲株BL5 6和BL61。对其培养条件进行了优化 ,在优化的培养条件下 ,生物量可以达到 83 0 6g L ,为初始培养条件下的 1 3 5倍。An excellent baker's yeast strain ZLTH-58 (MATa/α,leu-,FLO1) with hi gh biomass, high-sugar-tolerance and good flocculation was constructed by primar y screening, isolation of haploid, mutagenesis, cloning and expression of FLO1 gen e and hybridization. The results showed that strain ZLTH-58 had excellent prop e rties of the parental strains BL56 and BL61. The biomass of strain ZLTH-58 was 1 21 times of the parental strain BL56; The ability of high-sugar-tolerance o f st rain ZLTH-58 was higher than the parental strain BL61.The ability of flocculati o n of strain ZLTH-58 was better than the parental strains BL56 and BL61.The fact o rs that affected the biomass of strain ZLTH-58 were also detected. Biomass reac hed 83 06g/L under the optimal fermentation conditions, having a 1 35-fold im p rov ement. Strain ZLTH-58 is also stable in genetics characters by analysis of gene tic stability and it has the potential for use in industrial processes.

关 键 词：絮凝性 面包酵母 选育 絮凝基因表达 杂交 耐高糖 生物量 

分 类 号：Q933[生物学—微生物学] TS213.21[轻工技术与工程—粮食、油脂及植物蛋白工程]