超高水溶性麦芽糖基β-环糊精的生物反向合成  

Synthesis of Highly Soluble Maltosyl(α-1→6)β-Cyclodextrin Through the Reverse Reaction of Pullulanase

作　　者：周博[1] 方燕[1] 常辉[1] 杭锋[1] 曹新志[1] 徐学明[1] 

机构地区：[1]江南大学食品学院,江苏无锡214036

出　　处：《无锡轻工大学学报（食品与生物技术）》2003年第6期31-34,88,共5页Journal of Wuxi University of Light Industry

摘　　要：以麦芽糖、β 环糊精为原料,采用普鲁蓝酶生物反向合成得到超高水溶性的麦芽糖基(α 1→6)β 环糊精.试验测定,在pH4.0～4.2,温度58～60℃,酶量220～308U/gβ CD,麦芽糖与β 环糊精的摩尔比为7∶1～8∶1,反应时间35～38h的条件下,环糊精转化率可达到40%以上.Maltosyl β-Cyclodextrin was synthesized from maltose and β-cyclodextrin in a reverse reaction using pullulanase. More than 40% of maltosyl β-cyclodextrin was obtained under the following conditions: molar ratio of maltose to β-cyclodextrin 7:1 to 8:1, the amount of pullulanase 220～308 U/g β-cyclodextrin, the pH 4.0～4.2, and the reacting temperature 58～(60 ℃), respectively.

关 键 词：麦芽糖 Β-环糊精 普鲁蓝酶 生物反向合成 超高水溶性麦芽糖基β-环糊精 

分 类 号：TS202.3[轻工技术与工程—食品科学]