营养因素对乳酸乳球菌DL-203生长及其Nisin生物合成的影响  

Influence of nutrient factors on the growth of Lactoccus latis subsp. lactis DL-203 and its biosynthesis of Nisin.

作　　者：焦中高[1] 陈海昌[2] 刘茵[2] 

机构地区：[1]中国农业科学院郑州果树研究所,郑州450009 [2]大连轻工业学院食品与生物工程系,大连116001

出　　处：《食品工业科技》2003年第11期22-24,共3页Science and Technology of Food Industry

摘　　要：对Nisin高产菌株乳酸乳球菌DL—203的营养要求及其 对Nisin生物合成的影响进行了研究。结果表明,乳链菌 肽的生物合成与DL-203的生长呈极显著正相关,乳酸 乳球菌DL—203生长和合成Nisin的最佳碳源和氮源分 别为蔗糖和大豆蛋白胨,其适宜浓度均为15～20g/L。磷 酸盐对乳酸乳球菌DL—203生长及其Nisin生物合成具 有极大的促进作用,其中以Na_2HPO_4效果最好,其浓度 在224mmol/L以下时对菌体生长和Nisin生物合成具有 较大的促进作用。ln this paper the nutrition requirements of Lactococcus lactis Subsp. lactis DL -203 and its influence on the biosynthesis of Nisin were studied. The relationship between growth of DL-203 and biosynthesis of Nisin showed their significant positive correlation.The optimum carbon source and nitrogen souce were sucrose and soybean peptone respectively, and the optimum concentration for each was 1.5 ~2.0% (w/v). Phosphate could promote the growth of DL-203 and its biosynthesis of Nisin significantly, and Na2HPO4 showed the best effect, it showed better promotive effects on the growth of DL-203 and its biosynthesis of Nisin when the concentration was below 224 mmol/L.

关 键 词：生物合成 营养要求 乳酸乳球菌 NISIN 乳链菌肽 DL-203 

分 类 号：TS202.3[轻工技术与工程—食品科学]