黄桃水溶性多糖的抗肿瘤作用及清除自由基、提高免疫活性研究  

The Study on The Antitumor Activity and Scavenging Free Radical and Immune Effect of The Water-soluble Polysaccharides from A.Persica.L.var.seleropersica

作　　者：陈留勇[1] 孟宪军[1] 贾薇[2] 孔秋莲[3] 郑玉生[3] 章丽丽[3] 

机构地区：[1]沈阳农业大学食品学院,沈阳110161 [2]上海农业科学院食用菌所,上海201106 [3]上海市农业科学院农产品保鲜加工中心,上海200437

出　　处：《食品科学》2004年第2期167-170,共4页Food Science

摘　　要：从黄桃果肉中提取出两种水溶性多糖HTP1和HTP2,本文研究了HTP1和HTP2对自由基的清除作用,以及以小鼠为动物模型研究了它们在抗肿瘤及提高免疫力方面的功效,实验结果表明,HTP1和HTP2对羟基自由基(·OH)均有显著的清除作用,其IC50分别为270.92μg/ml和4.361mg/ml。HTP1对超氧阴离子自由基(O2)有明显清除作用,其IC50约为401.3μg/ml,而HTP2无清除超氧阴离子自由基(O2)的能力。HTP1及HTP2各分别为100mg/kg、200mg/kg剂量ip×10qd方案,对小鼠Lewis肺癌足趾皮下接种的抑瘤率分别为54.69%、43.44%及47.08%、44.48%。对荷Lewis肺癌小鼠的NK及淋巴细胞转化的活性,HTP1和HTP2两个剂量组成均有较明显的促进和提高。The water soluble polysaccharides of peach were extracted in water, and then isolated by ethanol, through DEAE-sehparose cation exchange column chromatography and Sephacryl G-300 gel column chromatography, we got two polysaccha-rides HTP1 and HTP2. In this paper, we studied the Effect of Scavenging Free-radical of HTP1 and HTP2. The results showedthat they had a evident effect in scavenging ·OH, Their IC50 were 270.92μg/ml and 4.361mg/ml. HTP1 had a evident effectin scavenging O2-, the IC50 was 401.3μg/ml, but HTP2 had no effect on O2-. HTP1 and HTP2 had relatively high growth-inhibition activities against Lewis lung carcinoma and enhanced immune function of the mice, whice took it. HTP1 and HTP2were injected by C5BL/6 mice at two dosages of 100 mg/kg per day and 200 mg/kg per day, the inhibition rate of Lewis lungcarcinoma by subcutaneous inoculation to mice at toe were about 43.44%～54.69% and 44.48%～47.08% respectively.Meanwhile, HTP1 and HTP2 enhanced the activities of NK cells and lymphocyte activities of tumor-bearing mice.

关 键 词：黄桃 水溶性多糖 抗肿瘤作用 自由基 清除作用 动物实验 中草药 

分 类 号：R282.71[医药卫生—中药学]