酶法提取变性脱脂豆粕中蛋白质的研究  

Study on Protein Obtained from Denatured Soyben Meal with Proteinase

作　　者：刘芳[1] 王遂[1] 

机构地区：[1]哈尔滨师范大学化学系,黑龙江哈尔滨150080

出　　处：《食品科学》2004年第3期89-92,共4页Food Science

摘　　要：变性豆粕是由大豆浸油后,经高温脱溶所得。本文以高温变性脱脂大豆粕为原料,用正交实验法对变性豆粕在蛋白酶作用下的水解特性进行了深入研究。选用国产胰蛋白酶为水解酶对变性豆粕进行水解,研究了变性豆粕中蛋白质溶出率随温度、pH值、时间、底物浓度及用酶量的变化规律,找到了水解变性豆粕的最佳实验条件,为生产实践提供了基础数据。该研究结果对其它蛋白质原料的水解特性研究也具有参考价值。研究结果表明:胰蛋白酶水解高温变性豆粕的最佳条件为:温度50℃,时间6h,底物浓度11%,用酶量1000U/g,pH值8.0,在此条件下,变性豆粕中蛋白质可有69.34%水解溶出。所得蛋白质其水解度DH为9.0%,溶解度(用氮溶解指数NSI表示)为57.0%。The denatured soybean meal was obtained from the high temperature desolventized bean. At the same time thedenatured soybean meal hydrolyzed with proteinase was studied systematically by using orthogonal experiment method.Fer thedenatured soybean meal hydrolyzed with basel proteinase,the effets of temperature,pH value,time,substrate corcentraction andenzyme/substractrate during the hydrolysis were studied. The optimum conditions of the hydrolytic high temperature denaturedsoybean meal was obtained. The results provided the reference values for the study of hydrolysis of the other proteinmaterials.Results indicated that the optimum extrodting conditions of the denatured soybean meal by basic proteinase were asfollowings:temperature:50℃,time 6h,substract conurtraction 11.0%,enzyme/substract 10000U/g.In such conditions 69.34%protein in denatured soybean meal would be hydrolyzed and digested.

关 键 词：酶法提取 蛋白质 水解 变性脱脂豆粕 胰蛋白酶 

分 类 号：TS214.2[轻工技术与工程—粮食、油脂及植物蛋白工程]