从酵母细胞中分离纯化醇脱氢酶  

Separation and purification of alcohol dehydrogenase from yeast cell

作　　者：柳畅先[1] 吴士筠[1] 胡卫钊[1] 

机构地区：[1]中南民族大学化学与生命科学学院,湖北武汉430074

出　　处：《化学研究与应用》2004年第2期243-244,共2页Chemical Research and Application

摘　　要：In this paper Introduced the procedure for the separation and purification of alcohol dehydrogenase from yeast cell. The crude extract was treated by saturated ammonium sulfate,further purified by Sephadex G-100 column and DEAE-cellulose ion exchange column chromatography. The recovery Was 2.5%,and purification-flod reached 26.2.In this paper Introduced the procedure for the separation and purification of alcohol dehydrogenase from yeast cell. The crude extract was treated by saturated ammonium sulfate,further purified by Sephadex G-100 column and DEAE-cellulose ion exchange column chromatography. The recovery Was 2.5%,and purification-flod reached 26.2.

关 键 词：酵母细胞 分离 纯化 醇脱氢酶 食品添加剂 生长 细胞破碎 

分 类 号：TS202.3[轻工技术与工程—食品科学] Q554.9[轻工技术与工程—食品科学与工程]