高效液相色谱-质谱法分离鉴定天然红心鸭蛋黄中的红色素  

Separation and Identification of Red Pigments in Natural Red Yolk of Duck's Eggs by HPLC-MS-MS

作　　者：刘良忠[1] 张民[1] 彭光华[1] 王海滨[1] 张声华[1] 

机构地区：[1]华中农业大学食品科技学院

出　　处：《色谱》2004年第3期197-201,共5页Chinese Journal of Chromatography

基　　金：国家博士点基金资助(No.20020504008).

摘　　要：采用薄层色谱、高效液相色谱 质谱 质谱(HPLC MS MS)及高分辨电子轰击质谱(EI MS)方法,分离鉴定了天然红心鸭蛋(简称红心蛋)蛋黄中红色素的分子组成和结构。以甲醇 水(体积比为99 5∶0 5)为流动相,用C18柱从红心蛋的红色素中分离出4个峰。光电二极管阵列检测器(PDA)扫描图显示,4个峰均为单峰,最大吸收波长分别为482,488,496,501nm。HPLC MS MS分析结果表明,红心蛋中的红色素的各个峰具有相同的相对分子质量(Mr=562),其二级质谱具有紫杉紫素(rhodoxanthin)的特征碎片峰。高分辨EI MS的结果显示,红心蛋红色素分子式为C40H50O2。研究结果表明,红心蛋中的红色素为一种含羰基的类胡萝卜素紫杉紫素,其中含有多种紫杉紫素的顺式异构体。The natural red yolk of duck's eggs is produced by the laying duck in the lake areas in southward of China. In the laying duck breading areas such as Honghu, Jianli, Xiantao, Tianmen and Hanchuan citys in Hubei Province, the culturists are used to feeding fresh pondweeds to the laying ducks. The yolk of duck's eggs is natural red with the chrominance reaching up to and/or above RCF (Roche Yolk Color Fan) 15. The red pigment components of natural red yolk of duck's eggs were separated and identified by thin layer chromatography (TLC), high performance liquid chromatography-mass spectrometry-mass spectrometry (HPLC-MS-MS) and high resolution electron impact-mass spectrometry (EI-MS). Four isomers of red pigments were separated by HPLC on a RP-C_(18) column with methanol-water (99.5∶0.5, v/v) as mobile phase. The λ_(max) of the four components were 482, 488, 496, 501 nm, respectively, and all of them were single peak on chromatogram. They had the same molecular mass (M_r=562), and had the same fragment peaks of MS^2 with rhodoxanthin. The molecular formula of red pigments was determined as C_(40)H_(50)O_2 by high resolution EI-MS. The results indicate that the red pigment is rhodoxanthin, and they are all cis-isomers of rhodoxanthin.

关 键 词：高效液相色谱 质谱 鉴定 红色素 蛋美 天然红心鸭蛋 

分 类 号：O658[理学—分析化学]