目的:探讨microRNA-29c对人胰腺癌细胞(As PC-1、Bx PC-3、PANC-1、MIA Pa Ca-2)生物学特性的影响。方法:培养1种人正常胰腺上皮细胞(HPDE)及4种人胰腺癌细胞(As PC-1、Bx PC-3、PANC-1、MIA Pa Ca-2),采用real-time PCR法观察5种细胞系...
supported by grants from the National Natural Science Foundation of China(81071775,81272659,81101621,81160311,81172064,81001068,81272425 and 81101870);National“Eleventh Five-Year”Scientific and Technological Support Projects(2006BAI02A13-402);Key Projects of Science Foundation of Hubei Province(2011CDA030);Research Fund of Young Scholars for the Doctoral Program of Higher Education of China(20110142120014)
BACKGROUND: Myeloid-derived suppressor cells (MDSCs) are heterogeneous cell types that suppress T-cell responses in cancer patients and animal models, some MDSC subpopulations are increased in patients with pancrea...
supported by grants from the National Natural Science Foundation of China(No.81160311 and No.81101621);the Outstanding Young Training Project of Science and Education of Guizhou Province;China(No.[2012]177);the Postdoctoral Science Foundation of China(No.2013M531983)
Summary: The expression levels of hypoxia-inducible factor lalpha (HIF-lct) and HIF-2a in pancreatic cancer (PC) and their association with clinicopathologic characteristics were investigated in order to elucidat...
目的:研究microRNA-100(miR-100)对人胰腺癌细胞MIA Pa Ca-2和CFPAC-1增殖及皮下成瘤能力的影响。方法:用miR-100慢病毒过表达载体感染MIA Pa Ca-2和CFPAC-1细胞,构建高表达miR-100细胞株;用平板克隆、CCK-8方法检测miR-100对人胰腺癌...