云南高校图书馆联盟文献共享服务平台- 国家自然科学基金(39830070)

国家自然科学基金(39830070): 作品数：32被引量：74H指数：6; 导出分析报告; 相关作者：袁建刚章祥荪彭小忠强伯勤张菊亮更多>>; 相关机构：中国科学院数学与系统科学研究院中国医学科学院中国协和医科大学中国医学科学院基础医学研究所中国医学科学院北京协和医学院更多>>; 相关期刊：《科学通报》《Acta Biochimica et Biophysica Sinica》《应用数学学报》《中国生物化学与分子生物学报》更多>>; 相关主题：CDNA克隆全长CDNA基因克隆原核表达更多>>; 相关领域：生物学理学医药卫生自动化与计算机技术更多>>

The cloning and preliminarily functional analysis of the human neurotrimin gene被引量：1: 《Science China(Life Sciences)》2004年第2期158-164,共7页LIU Jin, LI Guangtao, PENG Xiaozhong, LIU Bingyan, YIN Bin, TAN Xinyu, FAN Ming, FAN Wenhong, QIANG Boqin & YUAN Jiangang National Laboratory of Medical Molecular Biology, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences, School of Basic Medicine, Peking Union Medical College, National Human Genome North Center, Beijing 100005, China Department of Neuro- biology, Institute of Basic Medical Science, 27 Taiping Road, Beijing 100850, China; This work was supported by the National Program for Key Basic Research Project(G1998051002);the National High Technology Research and Development Program(2001 AA221041;2002BA711A01-03);the National Natural Science Foundation of China(Grant No.39830070).; Proteins of the immunoglobulin superfamily (IgSF) are involved in a variety of spe- cific cell-cell interactions in the developing nervous system. To identify and characterize new members of this protein family in hum...; 关键词：human neurotrimin (NTM) immunoglobulin superfamily (IgSF) GPI-ANCHOR neural cell adhesion molecules.

Patterns of mouse reticulon 3 mRNA and protein expression in the mouse central nervous system: 《Chinese Science Bulletin》2003年第19期2044-2049,共6页LIUJin HUANGXiaowei PENGXiaozhong YANGHongbo YINBin TANXinyu FANMing FANWenhong LIUBingyan QIANGBoqin YUANJiangang; This work was supprted by the National Key Basic Rescarch Project(Grant Nos.G1998051002 and 001cb510206);the National High Technology Research and Development Program(Grant Nos.2001AA221041 and 2002BA711A01-03);the National Natural Sciences Foundation of China(Grand No.39830070).; Reticulons (RTN) are endoplasmic reticulum- associated protein complexes, which are localized in the endoplasmic reticulum (ER) and identified as markers for neuroendocrine differentiation. At least four different RTN...; 关键词：MRNA 蛋白质中枢神经系统鼠模型 RTN 原位杂交免疫组织化学反应神经内分泌特效蛋白质

Some Properties of a Measure of Information Discrepancy被引量：7: 《Systems Science and Systems Engineering》2002年第4期397-408,共12页FANGShun-lan FANGWei-wu; Thiswork issupported by National Natural Science Foundation of China(90 10 30 33,39830 0 70 ) andNKBRSF (G19980 30 60 ); Based on a group of axioms, a measure of information discrepancyamong multiple information sources has been introduced in and it possesses some peculiar properties compared with other measures of information discrepa...; 关键词：information discrepancy DISTANCE χ2 function Shannon entropy K-L entropy

Construction of human and mouse brain cDNA libraries and isolation of full-length cDNAs: 《Progress in Natural Science:Materials International》2002年第6期477-480,共4页ZHOU Yan, HU Songnian, HUANG Xiaowei, ZHOU Haijun, TAN Xinyu, PENG Xiaozhong, QIANG Boqin and YUAN Jiangang( State Key Laboratory of Medical Molecular Biology, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences & Peking Union Medical College, Chinese National Human Genome Center, Beijing 100005, China); Supported by the Major State Basic Research Development Program of China (G19988051000) ;the National Natural Science Foundation of China (Grant No. 39830070); cDNA libraries from aborted human 3-month fetal brain,adult rat and mouse brain were constructed by using a yZAP express cDNA library construction kin.Low molecular weight fragments of the second strand cDNASA were re...; 关键词：CDNA library brain EST.

Reductase activity of human thioredoxin-like protein and its effects on cell apoptosis: 《Progress in Natural Science:Materials International》2002年第9期713-716,共4页JIN Jian, ZHOU Yan, HU Xiaoyan, SHI Ning, HAN Chunyu, XU Caimin, QIANG Boqin,YUAN Jiangang and PENG Xiaozhong(National Laboratory of Medical Molecular Biology, Institute of Basic Medical Sciences, Peking Union Medical College & Chinese Academy of Medical Sciences, National Human Genome Center, Beijing 100005, China); Supported by the National Natural Science Foundation of China (Grant No. 39830070);the Major State Basic Research Development Program of China (G1998051002) ; National High Technology Research and Development Program (2001AA221041); Thioredoxin is a ubiquitous dithiol oxidoreductase found in many organisms and involved in numerous biochemical processes. Human thioredoxin-like protein (hTRXL) is differentially expressed at different development st...; 关键词：HUMAN thioredoxin-like gene (hTRXL) FETAL cerebrum reduction assay apoptosis.

人的类硫氧还蛋白还原酶活性的测定及其对细胞凋亡的影响: 《自然科学进展》2002年第8期848-851,共4页金鉴周彦胡晓燕石宁韩春雨许彩民强伯勤袁建刚彭小忠; 国家"八六三";"九七三"项目(G199805102);国家自然科学基金(批准号:39830070); 在对人的类硫氧还蛋白基因(hTRXL)进行了Northern印迹法鉴定的基础上表达了该基因产物,并对hTRXL蛋白的功能进行了研究,hTRXL胰岛素还原酶活性实验显示全长hTRXL和其N端结构域(hTRXL-N)都具有胰岛素还原酶活性;C端区域(hTRXL-C)不具有...; 关键词：人类硫氧还原蛋白基因胎脑淋巴瘤细胞细胞凋亡重组质粒转染乳癌细胞系胰岛素 Northern印迹法

人新的PLP基因全长cDNA的克隆与分析: 《自然科学进展》2002年第7期750-753,共4页黄晓玮周海军陈建和周严李光涛彭小忠袁建刚强伯勤; 国家自然科学基金(批准号:39830070);国家"八六三"计划;国家重点基础研究发展规划(批准号:1998051002)资助项目; 在批量分离与脑发育相关的基因克隆时,从人3个月胎脑cDNA文库中得到一全长2261bp的cDNA克隆,其开放性阅读框架编码一个含有551个氨基酸残基的蛋白质,此蛋白与一种推测的细胞结构调节蛋白parelemmin同源,故命名为PLP(paralemmin-like pro...; 关键词：结构调节蛋白细胞膜动力学 CDNA克隆人PLP基因 CDNA文库基因表达

人肿瘤坏死因子样蛋白全长cDNA的克隆及原核表达: 《中国医学科学院学报》2002年第3期246-249,共4页胡晓燕周严代芸陈建萌张斌彭小忠袁建刚强伯勤; 国家自然科学基金(39830070);国家高技术研究发展计划863重点项目基金(2001AA221041);国家重点基础性研究973项目基金(G1998051002)资助; 目的分离、克隆编码人肿瘤坏死因子样蛋白(TNFLP)的全长cDNA,并对其功能进行初步研究。方法从本室构建的λZAP表达胎脑文库中分离到编码TNFLP的全长cDNA。应用多种软件对该基因及其编码蛋白进行分析,寻找其同源蛋白,分析其亲、疏水性及...; 关键词：肿瘤坏死因子 -α 肿瘤坏死因子样蛋白原核表达基因克隆

人M961全长cDNA及剪接异构体的分离与克隆被引量：1: 《中国医学科学院学报》2002年第3期254-258,共5页张斌汪俊华陶波李光涛周严彭小忠袁建刚强伯勤; 国家自然科学基金(39830070);国家高技术研究发展计划863重点项目基金(2001AA221041);国家重点基础性研究973项目基金(G1998051002)资助; 目的分离、克隆人M96基因,并初步探索其在不同血细胞系中的表达。方法根据与鼠M96基因有较高同源性的人表达序列标签(EST)设计筛库引物,筛选成人睾丸及胎脑cDNA文库,采用BLAST及CLUSTALW等程序比较分析筛选到的阳性克隆;应用多细胞系杂...; 关键词：剪接异构体 M96基因 M961基因 M962基因 PHD锌指结构基因克隆基因分离白血病

ENC1的克隆,原核表达与数种细胞系表达谱分析被引量：1: 《中国生物化学与分子生物学报》2002年第5期605-608,共4页丰竹张斌刘瑾彭小忠袁建刚; 国家科委 86 3高科技计划 ( 2 0 0 1AA2 2 10 41);国家自然科学基金( 39830 0 70 );国家重点基础研究发展规划 973项目 (G19980 5 10 0 2 )资助~~; 以人 3月胎脑总RNA为模板 ,用RT PCR的方法得到了ENC1(ectoderm neuralcortex 1)基因的cDNA ,经测序证实该cDNA的长度为 180 0bp ,包含ENC1的完整编码区 .将之克隆入pGEX 4T 1载体构建重组表达质粒 ,转化大肠杆菌BL2 1表达 ,经Sepharose...; 关键词：ENC1 原核表达细胞系表达谱分析蛋白纯化神经细胞基因克隆

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