国家自然科学基金(20672045)

作品数：7被引量：11H指数：2
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相关作者：曹淑桂杜创王磊王智吉腾飞更多>>
相关机构：吉林大学中国医学科学院北京协和医学院更多>>
相关期刊：《高等学校化学学报》《Chemical Research in Chinese Universities》更多>>
相关主题：PURIFICATIONESCHERICHIA_COLIDOMAIN丙烯酸乙酯MICHAEL加成反应更多>>
相关领域：农业科学理学生物学医药卫生更多>>
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Analysis of a Critical Residue Determining Herbicide Efficiency Sensitivity in Carboxyltransferase Domain of Acetyl-CoA Carboxylase from Poaceae by Homology Modeling and Free Energy Simulation
《Chemical Research in Chinese Universities》2010年第5期816-821,共6页TAO Jin ZHAO Bo TIAN Xue-mei ZHENG Liang-yu CAO Shu-gui 
Supported by the National Natural Science Foundation of China(Nos.20802025, 30870539, 20432010 and 20672045)
Carboxyltransferase domain（CT） of acetyl-coenzyme A carboxylase（ACCase, EC 6.4.1.2） from a family of Poaceae is an important target of commercial herbicide APPs for controlling grass weed growth. As the abuse of A...
关键词：Molecular modeling Molecular dynamics Free energy calculation Herbicide resistance 
Refolding and Purification of Yeast Acetyl-CoA Carboxylases CT Domain Expressed as Inclusion Bodies in Escherichia coli
《Chemical Research in Chinese Universities》2009年第5期690-694,共5页YANG Xue-ying TAO Jin ZHENG Liang-yu WANG Rui-jian ZHAO Ke CAO Shu-gui 
Supported by the National Natural Science Foundation of China(Nos.20432010,20672045 and 30870539)
Acetyl-CoA carboxylase（ACCase） is a crucial enzyme in fatty acid synthesis, by regulating the first committed step in the process. Therefore, it is a potential target for the development of new compounds against obe...
关键词：Carboxyltransferase domain Expression Inclusion body REFOLDING 
Cloning, Expression and Purification of Wheat Acetyl-CoA Carboxylases CT Domain in E. coil
《Chemical Research in Chinese Universities》2008年第6期752-755,共4页WANG Rui-jian YANG Xue-ying ZHENG Liang-yu YANG Ye GAO Gui CAO Shu-gui 
Supported by the National Natural Science Foundation of China(Nos. 20432010, 20672045 and 30570405)
The entire gene of carboxyltransferase（CT） domain of acetyl-CoA carboxylase（ACCase） from Chinese Spring wheat（CSW） plastid was cloned firstly, and the 2.3 kb gene was inserted into PET28a^＋ vector and expressed...
关键词：Chinese Spring wheat Acetyl-CoA carboxylase Carboxyltransferase（CT） domain CLONING Expression PURIFICATION 
酶促尿嘧啶与丙烯酸乙酯Michael加成反应的研究被引量：5
《分子催化》2008年第2期172-176,共5页王磊 杜创 吉腾飞 王智 曹淑桂 
国家自然科学基金(批准号:20432010,20672045,30400081).
考察了4种水解酶在尿嘧啶与丙烯酸乙酯Michael加成反应中的催化效率.初步探讨了反应溶剂,反应温度,反应时间,底物配比,酶添加量等反应条件对该加成反应的影响.筛选得到最适反应条件,在该反应条件下,Michael加成反应产率可达61·2%.
关键词：酶 尿嘧啶 丙烯酸乙酯 MICHAEL加成 
古细菌Aeropyrum pernix K1超嗜热酯酶APE1547的稳定性被引量：2
《高等学校化学学报》2008年第1期109-112,共4页解桂秋 高仁钧 毕云枫 王中禹 刘娜 冯雁 曹淑桂 
国家自然科学基金(批准号:30400081,20432010和20672045)资助
研究了纯化的超嗜热酯酶APE1547的稳定性．结果表明，该酶的稳定性非常好，蛋白的质量浓度为0．4mg／mL时，90℃的半衰期为20h，0．2mg／mL时的半衰期为12h；而蛋白的质量浓度为0．04mg／mL时，保温2．5h时残余活力仍在50％以上．同时...
关键词：古细菌 超嗜热酯酶 热稳定性 AEROPYRUM PERNIX K1 
High-throughput Screening of the Enantioselectivity of Hyperthermophilic Mutant Esterases from Archaeon Aeropyrum pernix K1 for Resolution of (R,S)-2-Octanol Acetate被引量：1
《Chemical Research in Chinese Universities》2007年第3期319-324,共6页ZHANG Gui-rong GAO Ren-jun ZHANG Ai-jun RAO Lang CAO Shu-gui 
Supported by the National Natural Science Foundation of China(Nos30400081, 30570405 and 20672045);the Key Tech-nology Research and Development Program of China(No2004BA713D03-04)
To identify the desired hypertherrnophilic variants within a mutant esterase library for the resolution of （R, S）-2- octanol acetate, a simple, reliable, and versatile method was developed in this study. We built a ...
关键词：High-throughput screening ENANTIOSELECTIVITY Hyperthermophilic esterase Directed evolution 
Expression and Purification of Active Recombinant Human Nerve Growth Factor from Escherichia coli被引量：3
《Chemical Research in Chinese Universities》2007年第2期237-240,共4页HE Ling-bing WANG Yan WANG Ge CHEN Chao CAO Shu-gui 
the National Natural Science Foundation of China(Nos 20432010, 30570405 and 20672045)
Introduction Nerve growth factor （NGF） was first discovered and purified by Rita Levi-Montalcini and Stanley Cohen in the 1950s. It represents the first cellular growth factor ever discovered and involved in the gl...
关键词：Nerve growth factor Fusion protein PURIFICATION Biological activity 
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