云南高校图书馆联盟文献共享服务平台- 国家自然科学基金(20932001)

国家自然科学基金(20932001): 作品数：13被引量：6H指数：1; 导出分析报告; 相关作者：杨振军张礼和马元王晓锋黄野更多>>; 相关机构：北京大学更多>>; 相关期刊：《Journal of Chinese Pharmaceutical Sciences》《Science China Chemistry》《中国科学：化学》《Chinese Chemical Letters》更多>>; 相关主题：SIRNAOLIGONUCLEOTIDESPEPTIDEBIOLOGICALAMINO更多>>; 相关领域：医药卫生生物学理学化学工程更多>>

Transfection of 3′,3′′-bis-peptide-siRNA conjugate by cationic lipoplexes mixed with a neutral cytosin-1-yl-lipid: 《Journal of Chinese Pharmaceutical Sciences》2017年第10期719-726,共8页杨梦依孙晶王超张艳芬张礼和杨振军; The National Natural Science Foundation of China(Grant No.21778006 and 20932001);the Ministry of Science and Technology of China(Grant No.2012AA022501); Cationic lipids have been applied to siRNA delivery for tumor therapeutics. However, the excess positive charges of these nanoplexes may lead to high cytotoxicity and nonnegligible immunogenicity both in vitro and in ...; 关键词：3＇ 3＂-Bis-peptide siRNA conjugate Gemini-like cationic lipid Cytosin-1-yl-lipid Melanoma therapy

Evaluation of the intracellular trafficking of siRNAs in A375 cells by confocal laser scanning microscopy: 《Journal of Chinese Pharmaceutical Sciences》2016年第12期859-868,共10页Yiping Diao Jing Sun Mengyi Yang Bo Xu Lihe Zhang Zhenjun Yang; Ministry of Science and Technology of China(Grant No.2012AA022501,2012CB720604);the National Natural Science Foundation of China(Grant No.20932001,81302626); Investigation intracellular trafficking of siRNAs following their delivery to cells is of great interest to elucidate dynamics of siRNA in cytoplasm. In this study, we present a novel confocal laser scanning microsco...; 关键词：siRNA/CLD complex Endosomal escape Cytoplasmic distribution Confocal laser scanning microscopy

siRNA缀合物的研究进展被引量：1: 《中国科学：化学》2016年第7期633-642,共10页孙晶杨振军; 国家自然科学基金重点课题(编号:20932001);国家重点基础研究发展计划(编号:2012CB720604);国家高技术研究发展计划(编号:2012AA022501)项目资助; 小干扰RNA(small interfering RNA,si RNA)能够对靶m RNA进行高效性、高特异性转录后调控,具有可传递性等特点,已被用于多种疾病的治疗研究.但其自身存在的诸多缺陷(如稳定性差、难以跨膜等)限制了它在临床上的广泛应用.对核苷酸单元进...; 关键词：小干扰RNA siRNA缀合物化学合成基因沉默

茎环引物实时定量PCR技术用于检测异核苷修饰的siRNA的可行性研究被引量：1: 《中国科学：化学》2015年第7期710-718,共9页王雨斯马元刘欣洁张礼和杨振军; 国家重点基础研究发展计划(2012CB720604);国家高技术研究发展计划(2012AA022501);国家自然科学基金(20932001)资助; 本文考察了茎环引物实时定量PCR技术用于定量检测D-/L-异核苷(D-/L-iso NA)修饰的siRNA的可行性.根据阈值循环数(Ct值)及异核苷修饰的siRNA模板的初始浓度的对数值(lgC)绘制了标准曲线,与siRNA的标准曲线进行对比,发现D-异尿苷(D-isodU...; 关键词：茎环引物实时定量PCR 异核苷绝对定量双标准曲线法

Serum stability enhancement of siRNA caused by peptide conjugation at 3'-terminus of sense strand: 《Journal of Chinese Pharmaceutical Sciences》2014年第4期215-219,共5页邹朗黄野王晓锋马元刘洋关注张礼和杨振军; Ministry of Science and Technology of China(Grant No.2012CB720604);National Natural Science Foundation of China(Grant No.20932001); RNA interference has been widely used for gene therapy of various infectious diseases and malignant tumors. However, its poor stability in serum has limited further clinic application. Here, we found that stability of...; 关键词：SIRNA Serum stability Peptide conjugation NUCLEASES

Loss of silencing activity caused by 5′-terminal modification with D-/L-isonucleotide(isoNA) or locked nucleic acid(LNA) could not be restored by 5′-terminal phosphorylation被引量：1: 《Science China Chemistry》2014年第2期329-334,共6页HUANG Ye CHEN Zhuo WANG Zhuo LI YaTing CHEN Yue YANG ZhenJun ZHANG LiHe; supported by the National Natural Sciences Foundation of China(20932001);the Program of the Ministry of Science and Technology of China(2009ZX09503,2012AA022501,2012CB720604); In this study,a series of 5′-phosphorylated siRNAs with D-/L-isonucleotide(isoNA)or locked nucleic acid(LNA)incorporated at the 5′-terminus were synthesized.It was found that after incorporating either isoNA or LNA ...; 关键词：isonucleotide locked nucleic acid （LNA） 5＇-terminal phosphorylation

Synthesis and biological evaluation of peptide-siRNA conjugates with phosphodiester unit as linker: 《Science China Chemistry》2013年第11期1542-1549,共8页WANG XiaoFeng HUANG Ye LIU Yang CHEN Yue JIN HongWei ZHENG Yi DU Quan YANG ZhenJun ZHANG LiHe; supported by the National Natural Science Foundation of China(20932001);the Ministry of Science and Technology of China(2012AA022501);the National Key Basic Research Program of China(2012CB720604); In this paper,a series of peptide-siRNA conjugates with phosphodiester unit as the linker targeting to Cdc2 gene were synthesized by solid phase stepwise strategy.The conjugation of peptides at either 3’-terminus of ...; 关键词：SIRNA PEPTIDE conjugate solid synthesis

Solid phase synthesis of peptide–siRNA conjugates containing disulfde bond unit: 《Chinese Chemical Letters》2013年第10期873-876,共4页Xiao-Feng Wang Xian-Tao Yang Yue Chen Yang Liu Lang Zou Zhen-Jun Yang Li-He Zhang; supported by the National Natural Science Foundation of China(No.20932001);the Ministry of Science and Technology of China(Nos.2012CB720604,2012AA022501); A disulfide-modified nucleoside was designed and synthesized. After loading the modified nucleoside on controlled pore glass （CPG）, solid phase synthesis strategy was used to prepare peptide-oligonucleotide conjugat...; 关键词：siRNA Solid synthesis Disulfide bond Peptide Conjugate

Development of a high-content screening method to evaluate the cellular population-based biological activity of new siRNA delivering reagent: 《Journal of Chinese Pharmaceutical Sciences》2013年第6期467-474,共8页李雅婷郑宜潘德林徐波武芸杨振军张礼和; Ministry of Science and Technology of China(Grant No.2012CB720604);NSFC(Grant No.20932001); Normally, cellular responses to modified siRNAs or new siRNA delivery systems have been studied in group cell behavior by PCR, western blotting and fluorescence microscopy. In this study, we present a novel high-conte...; 关键词：High-content screening siRNA delivery system Gene knock-down Cellular population ERK signaling pathway

Modification of oligonucleotides by isonucleosides incorporation and peptides conjugation: 《Journal of Chinese Pharmaceutical Sciences》2012年第6期499-508,共10页黄野王晓锋杨振军张礼和; National Natural Science Foundation (Grant No.20932001);the Ministry of Science and Technology (Grant No.2012CB720604); Synthetic oligonucleotides including antisense oligonucleotides and siRNA have shown promising therapeutic potential.However,to realize the therapeutic potential of synthetic oligonucleotides,many obstacles have to be...; 关键词：Synthetic oligonucleotide Isonucleoside Cell penetrating peptide SIRNA

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