Supported by the National Natural Science Foundation of China(No.20772046).
The aminopeptidase gene from thermophilic archaea Sulfolobustokodaii was cloned and expressed in Escherichia coli BL21 codon-plus(DE3). To overexpress the aminopeptidase, the vector pET32a was constructed, in which ...
Supported by the National Natural Science Foundation of China(Nos.31070708,21172093 and 20772046);the Natural Science Foundation of Jilin Province,China(No.201115038)
The regioselective acylation of quercetin catalyzed by a novel thermophilic esterase(APE1547) from the archaeon Aeropyrum pernix K1 was successfully conducted in organic solvents.The effects of acyl donor,substrate ...
Supported by the National Natural Science Foundation of China(Nos.30400081 and 20772046)
The gene of enzyme(Ape1547) was cloned from hyperthermophilic archaeon Aeropyrum pernix K1 and expressed in Escherichia coil.The effect of calcium cation on the properties of Ape1547 was studied.Ape1547 exhibits bot...