云南高校图书馆联盟文献共享服务平台- 国家重点基础研究发展计划(2009CB918600)

国家重点基础研究发展计划(2009CB918600): 作品数：11被引量：15H指数：2; 导出分析报告; 相关作者：徐殿胜姜凌刘买利杨忠曹春阳更多>>; 相关机构：中国科学院复旦大学华东理工大学北京化工大学更多>>; 相关期刊：《物理》《生物物理学报》《中医学报》《化学学报》更多>>; 相关主题：DNA核磁共振SMALL-ANGLE寡核苷酸酶降解更多>>; 相关领域：生物学理学机械工程医药卫生更多>>

Small-angle X-ray scattering probe of intermolecular interaction in red blood cells: 《Chinese Physics C》2015年第3期83-86,共4页刘广峰王文佳徐建华董宇辉; Supported by National Basic Research Program of China(2009CB918600);National Natural Science Foundation of China(10979005); With high concentrations of hemoglobin （Hb） in red blood cells, self-interactions among these molecules could increase the propensities of their polymerization and aggregation. In the present work, high concentratio...; 关键词：small-angle X-ray scattering protein-protein interaction HEMOGLOBIN

Solution of the effects of twinning in femtosecond X-ray protein nanocrystallography: 《Chinese Physics C》2013年第2期105-110,共6页周亮刘鹏董宇辉; Supported by National Natural Science Foundation of China (10979005);National Basic Research Program of China(2009CB918600); With the development of the XFEL （X-ray free electron laser）, high quality diffraction patterns from nanocrystals have been achieved. The nanocrystals with different sizes and random orientations are injected to the...; 关键词：coherent X-ray diffractive imaging XFEL oversampling technique protein nanocrystallography multiple-common-lines

Structural insight into substrate recognition by histone demethylase LSD2/KDM1 b被引量：2: 《Cell Research》2013年第2期306-309,共4页Fei Chen Huirong Yang Zhenghong Dong Jian Fang Ping Wang Tingting Zhu Wei Gong Rui Fang Yujiang Geno Shi Ze Li Yanhui Xu; We thank staff members of beamline BL17U at SSRF （Shang-hai Synchrotron Radiation Facility, China） for assistance in data collection, and staff members of Biomedical Core Facility, Fudan University for their help on mass spectrometry analyses. This work was supported by grants from the National Basic Research Program of China （2011CB965300 and 2009CB918600）, the Na-tional Natural Science Foundation of China （31270779, 31030019, 11079016 and 30870493）, and Fok Ying Tung Education Founda- tion （20090071220012）. This work was also supported by a grant from the National Institutes of Health （5 R01 GM078458） to YGS.; Dear Editor, Histone methylation is a reversible histone post-translational modification that plays an important role in various chromatin-based processes, including chromatin structure remodeling, transcription, and ...; 关键词：组蛋白甲基化结构洞染色质结构识别底物翻译后修饰 DNA修复组蛋白H3

Direct demodulation method for heavy atom position determination in protein crystallography被引量：1: 《Chinese Physics C》2013年第1期133-137,共5页周亮刘忠川刘鹏董宇辉; Supported by National Natural Science Foundation of China(10979005);National Basic Research Program of China(2009CB918600); The first step of phasing in any de novo protein structure determination using isomorphous replacement （IR） or anomalous scattering （AD） experiments is to find heavy atom positions. Traditionally, heavy atom posit...; 关键词：direct demodulation method heavy atom sites difference Patterson map protein crystallography

Structural insights into DndE from Escherichia coil B7A involved in DNA phosphorothioation modification被引量：7: 《Cell Research》2012年第7期1203-1206,共4页Wei Hu Chengkun Wang Jingdan Liang Tianlong Zhang Zhongpei Hu Zhijun Wang Wenxian Lan Fang Li Houming Wu Jianping Ding Geng Wu Zixin Deng Chunyang Cao; Acknowledgments This work was supported by National Basic Research Program of China （2009CB918600 and 2011 CB966300）, National Natural Science Foundation of China （20872169, 20905074 and 20921091）, National New Drug Design Program from Ministry of Health of China （2011ZX09506）, and Pujiang Talents Awards from Science and Technology Commission of Shanghai Municipality （08P J14117）. We thank Prof Jianhua He at the beamline BL 17U at SSRF （China） for assistance in data collection.; Dear Editor, DNA phosphorothioate （PT） modification, originally developed as an artificial tool to stabilize oligodeoxynucleotides against nuclease degradation , was recently found to be incorporated with sulfur in...; 关键词：DNA 大肠杆菌修改结构寡核苷酸生理变化磷酸酯酶降解

MMP-12蛋白包涵体复性时折叠状态核磁共振图谱与荧光光谱研究被引量：2: 《化学学报》2012年第4期453-458,共6页张琳琳冯延叶蓝文贤曹春阳徐殿胜杨忠; 国家重点基础研究发展计划(973计划)(No.2009CB918600)资助项目~~; MMP-12是癌症治疗药物靶标.为了更好研制新药,需要大量制备MMP-12,但MMP-12在大肠杆菌中以包涵体形式表达.因此如何优化蛋白复性过程是大量获取MMP-12蛋白的关键.采用核磁共振、稳态荧光法、外源性ANS(8-anilinol-naphthalenesulfonic a...; 关键词：核磁共振稳态荧光 ANS荧光探针 MMP-12 复性折叠

Solution conformation of the response regulator proteins from Deinococcus radiodurans studied by SAXS被引量：1: 《Chinese Physics C》2011年第10期974-977,共4页李丽琴刘颖刘鹏董宇辉; Supported by National Natural Science Foundation of China(10979005);National Basic Research Program of China(2009CB918600); In this paper the solution conformation of the response regulator proteins from Deinococcus radiodurans was studied by small-angle X-ray scattering （SAXS）. The SAXS curves of Dr-rrA in solutions were obtained at Bea...; 关键词：small-angle X-ray scattering response regulator Dr-rrA solution conformation

大肠杆菌DNA复制相关蛋白PriC的初步研究: 《中医学报》2011年第6期689-692,共4页孔敏敏李艳华董宇辉王文雅袁其朋; 国家自然科学基金(编号:10979005);国家基础研究项目(编号:2009CB918600); 目的:对大肠杆菌DNA复制相关蛋白PriC进行研究。方法:构建重组表达载体pET21a-PriC及pET21a-PriC1-101,并进一步诱导表达纯化。利用Pulldown手段将pET21a-PriC-notag与pET28at-plus-PriB分别转化到大肠杆菌BL21(DE3)中,诱导表达及共纯...; 关键词：大肠杆菌 DNA 蛋白PriC

核磁共振技术在生物研究中的应用被引量：2: 《物理》2011年第6期366-373,共8页姜凌刘买利; 国家自然科学基金(批准号:20921004;90813017);国家重点基础研究发展计划(批准号:2009CB918600)资助项目; 核磁共振(nuclear magnetic resonance,NMR)是以原子核自旋的共振跃迁为探测对象的谱学方法.当自旋量子数不为零的原子核处于外磁场中时,会引起能级的Zeeman分裂.若再施加能量等于Zeeman能级差的射频场,则会诱发原子核自旋的共振跃迁,...; 关键词：核磁共振蛋白质溶液结构蛋白质动力学药物识别

Instrumentation of coherent X-ray imaging and the primary experimental results: 《Chinese Physics C》2010年第2期224-226,共3页许锐刘超培; Supported by 973 Program of the Ministry of Science and Technology (2009CB918600); Coherent X-ray microscopy has advanced towards higher-energy, more brilliant sources over the past decade since its demonstrations, and many advancements have been made towards optimizing this imaging technique. Here ...; 关键词：CXDM OVERSAMPLING image reconstruction phase retrieval

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