SITE-SPECIFIC

作品数：120被引量：357H指数：8
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Probing the active sites of site-specific nitrogen doping in metal-free graphdiyne for electrochemical oxygen reduction reactions被引量：7
《Science Bulletin》2020年第1期45-54,M0004,共11页Xingzhu Chen Wee-Jun Ong Zhouzhou Kong Xiujian Zhao Neng Li 
financial supports by the Young Scientists Fund of the National Natural Science Foundation of China (11604249);the Fok Ying-Tong Education Foundation for Young Teachers in the Higher Education Institutions of China (161008);the Foundation of the State Key Laboratory of Optical Fiber and Cable Manufacture Technology (SKLD1602);the State Key Laboratory of Refractors and Metallurgy (G201605), the Fundamental Research Funds for the Central Universities (2019-III-034);the Research Board of the State Key Laboratory of Silicate Materials for Architectures;financial supports and grants from Xiamen University Malaysia,the Xiamen University Malaysia Research Fund (XMUMRF/2019-C3/ IENG/0013)
The development of highly active and low-cost catalysts for electrochemical reactions is one of the most attractive topics in the renewable energy technology.Herein,the site-specific nitrogen doping of graphdiyne(GDY)...
关键词：Graphdiyne(GDY) Site-specific nitrogen doping Metal-free catalysts Oxygen reduction reaction(ORR) 
Direct site-specific treatment of skin cancer using doxorubicin-loaded nanofibrous membranes被引量：5
《Science Bulletin》2018年第2期92-100,共9页Mengyu Guo Guoqiang Zhou Zhu Liu Jing Liu Jinglong Tang Yating Xiao Wenshi Xu Ying Liu Chunying Chen 
supported by the Project Electro Med (11115313) from the Danish Council for Strategic Research;the National Science Fund for Excellent Young Scholars (31622026);the National Natural Science Foundation of China (U1532122, 21320102003, 21471044);the National Key Research and Development Plan (2016YFA0201600, 2016YFA0203204);the National Science Fund for Distinguished Young Scholars (11425520);Youth Innovation Promotion Association of the Chinese Academy of Sciences (2014031)
Doxorubicin （DOX） is widely used in cancer therapy. However, its application is sometimes limited by its adverse cardiotoxicity and delivery pathways. In our study, we prepared a topical implantable delivery device ...
关键词：Core-shell nanofiberMelanomaElectrospinningControlled drug release 
Site-specific uv crosslinking of minihelix DNA and TrpRS from Bacillus subtilis
《Chinese Science Bulletin》2001年第16期1380-1383,共4页Xu, F Jiang, G Zhang, Y Zhu, LF Jin, YX Wang, DB 
This work was supported by the National Natural Science Foundation of China (Grant No. 39730120) ; the Chinese Academy of Sciences (Grant No. KSCX 2-2-04).
In order to investigate the recognition mechanism and the relationship between structure and function of minihelix DNA with Tryptophanyl-tRNA Synthetase (TrpRS), TrpRS from Bacillus Subtilis was purified. Four minihel...
关键词：UV CROSSLINKING minihelix DNA TrpRS discriminator. 
Establishment and application of both FLP and Cre site-specific recombination systems at the same position in the genome被引量：1
《Chinese Science Bulletin》2000年第5期456-460,共5页Jilong Chen Jie Liu Yulian Zhang Zhiwei Chen Zhenhua Jin 
Both FRT-FRT and LoxP-LoxP sites that are the target sepuences of site-specific recombinases have been constructed in a vector, called C4LFY, using the recombinant DNA technigue. C4LFY also contains P elements, 2 exon...
关键词：SITE-SPECIFIC recombination TRANSGENE gene expression and regulation cis-acting elements. 
Co-transformation to tobacco of Cre/lox site-specific recombination system and its precise recombination
《Chinese Science Bulletin》2000年第6期540-543,共4页Lei Li Songmei Liu Yuanlei Hu Zhongping Lin 
For the temporally and spatially regulated expression of the barnase gene in plant, two kinds of plasmids with ere gene and its directly repeat recognition sites lox from bacteriophage P1 were constructed and co-trans...
关键词：CRE/LOX SITE-SPECIFIC recombination system TOBACCO CO-TRANSFORMATION PRECISE recombination. 
Mutational effect of the “-35” element of sorghum psbA gene promoter被引量：1
《Chinese Science Bulletin》1999年第16期1474-1480,共7页Wu, NH Fan, TS Ren, DL 
Mutations of the first position T and the third position G in TTGACA, the ' - 35' element of sorghum psbA gene promoter, were induced using chemically synthesized 20 nt oligonucleotide primer. Three mutants were produ...
关键词：SORGHUM ( SORGHUM valgare P.) PSBA gene 5’-noncodlng region SITE-SPECIFIC MUTAGENESIS expression control. 
STUDY ON MULTI-POINT SITE-SPECIFIC MUTAGENESIS——DOUBLE-POINT SITE-SPECIFIC MUTAGENESIS FOR MODIFYING α-HUMAN ATRIAL NATRIURETIC PEPTIDE GENE
《Chinese Science Bulletin》1990年第3期232-238,共7页杨迪 谢毅 施文 闵永洁 王启松 
Although oligonucleotide-directed mutagenesis has been widely applied in the field of molecular biology, this method has some disadvantages: It is time-consuming and overelaborate, besides only a single base mutation ...
关键词：SITE-SPECIFIC MUTAGENESIS double-point SITE-SPECIFIC MUTAGENESIS α-human atrial NATRIURETIC peptide. 
DOUBLE POINTS SITE-SPECIFIC MUTATION OF A GENE FOR α-HUMAN CALCITONIN GENE RELATED PEPTIDE
《Chinese Science Bulletin》1989年第20期1758-1758,共1页杨迪 闵永洁 施文 陈晖 王启松 
Oligonucleotide directed mutagenesis is one of the fundamental methods in the study of protein engineering. The methods previously described have their own drawbacks, for instance, they are time-consuming and can only...
关键词：consuming instance MUTAGENESIS directed MUTATION annealing DNA SAVING POLYMERASE 代军 
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