KNOCK-IN

作品数：26被引量：105H指数：5
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相关领域：生物学医药卫生更多>>
相关作者：王红胡静姜元于翠娟吴智群更多>>
相关机构：吉林大学陕西师范大学广西大学国立中兴大学更多>>
相关期刊：《Journal of Integrative Agriculture》《Translational Neurodegeneration》《Genomics, Proteomics & Bioinformatics》《Neuroscience Bulletin》更多>>
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An App knock-in rat model for Alzheimer’s disease exhibiting Aβand tau pathologies,neuronal death and cognitive impairments被引量：14
《Cell Research》2022年第2期157-175,共19页Keliang Pang Richeng Jiang Wei Zhang Zhengyi Yang Lin-Lin Li Makoto Shimozawa Simone Tambaro Johanna Mayer Baogui Zhang Man Li Jiesi Wang Hang Liu Ailing Yang Xi Chen Jiazheng Liu Bengt Winblad Hua Han Tianzi Jiang Weiwen Wang Per Nilsson Wei Guo Bai Lu 
This work was supported by the National Key Research and Development Program of China(2017YFE0126500);the National Natural Science Foundation of China(81861138013,81501105,31730034);Beijing Advanced Innovation Center for Human Brain Protection,Shenzhen Science Technology and Innovation Commission(JCYJ20170411152419928 and JCYJ20180508152240368);Beijing Municipal Science&Technology Commission(Z151100003915118)to B.L.,W.G.;K.P.Strategic Priority Research Program of Chinese Academy of Science(NO.XDB32030200)to HH.We acknowledge funding from Hållstens forskningsstiftelse,Swedish Research Council,Swedish Brain foundation,Swedish Alzheimer foundation,Margaretha af Ugglas Foundation,Sonja Leikrans donation,China Scholarship Council and H2020-MSCA-ITN-2019#860035(R.J.,M.S.,S.T.,J.W.,B.W.and P.N.).We thank Takaomi Saido and Takashi Saito at RIKEN Center for Brain Science,Japan for providing the App knock-in mice to Department of Neurobiology,Care Sciences and Society,Division of Neurogeriatrics,Karolinska Institutet.We thank Dr.P.Davies(Albert Einstein College of Medicine,Bronx,NY)for antibodies Alz-50,and MC-1,Dr.Jianzhi Wang(Tongji Medical College,Huazhong University&Technology,Wuhan,Hubei)for APP/PS1 mice frozen brain sections,Dr.Chuanzhou Li and Dr.Hao Li(Tongji Medical College,Huazhong University&Technology,Wuhan,Hubei)for P301 mice frozen brain sections,Dr.Mengchao Cui(College of Chemistry,Beijing Normal University,Beijing)for P301 mice paraffin brain sections,and Xaomin Wang(Capital Medical University,Beijing)for 5×FAD mice frozen brain sections.We thank the Imaging Core Facility,the Technology Center for Protein Sciences(Tsinghua University)for assistance on confocal imaging and Imaris software data analysis.We also thank Dr.Yichang Jia(School of Medicine,Tsinghua University,Beijing)for the discussion of gene editing strategy and Dr.Guhan Nagappan for manuscript discussion and editing.CRISPR/Cas9 manipulation was done by a CRO company(Beijing Biocytogen Co.,Ltd).
A major obstacle in Alzheimer’s disease(AD)research is the lack of predictive and translatable animal models that reflect disease progression and drug efficacy.Transgenic mice overexpressing amyloid precursor protein...
关键词：TAU AΒ ALZHEIMER 
Generation of knock-in cynomolgus monkey via CRISPRJCas9 editing被引量：11
《Cell Research》2018年第3期379-382,共4页
Dear Editor, Genetically modified monkey models that exhibit hu- man disease symptoms are versatile tools for studying human disease mechanisms and advancing therapeutic treatments [1]. However, generation of gene-ta...
关键词：猴子 编辑 疾病症状 mRNA 繁殖周期 直接注射 治疗学 基因 
Homology-mediated end joining-based targeted integration using CRISPR/Cas9被引量：33
《Cell Research》2017年第6期801-814,共14页Yao, Xuan Wang, Xing Hu, Xinde Liu, Zhen Liu, Junlai Zhou, Haibo Shen, Xiaowen Wei, Yu Huang, Zijian Ying, Wenqin Wang, Yan Nie, Yan-Hong Zhang, Chen-Chen Li, Sanlan Cheng, Leping Wang, Qifang Wu, Yan Huang, Pengyu Sun, Qiang Shi, Linyu Yang, Hui 
Targeted integration of transgenes can be achieved by strategies based on homologous recombination （HR）, mi- crohomology-mediated end joining （MMEJ） or non-homologous end joining （NHEJ）. The more generally used ...
关键词：homology-mediated end joining CRISPR/Cas9 monkey embryos NEURONS KNOCK-IN 
APP physiological and pathophysiological functions： insights from animal models被引量：5
《Cell Research》2012年第1期78-89,共12页Qinxi Guo Zilai Wang Hongmei Li Mary Wiese Hui Zheng 
The amyloid precursor protein （APP） has been under intensive study in recent years, mainly due to its critical role in the pathogenesis of Alzheimer＇s disease （AD）. β-Amyloid （Aβ） peptides generated from APP ...
关键词：Alzheimer＇s disease APP AΒ KNOCK-IN animal models 
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