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In vitro testicular organogenesis from human fetal gonads produces fertilization-competent spermatids被引量：14
《Cell Research》2020年第3期244-255,共12页Yan Yuan Laihua Li Qing Cheng Feiyang Diao Qiao Zeng Xiaoyu Yang Yibo Wu Hao Zhang Mingqian Huang Junqing Chen Quan Zhou Yunfei Zhu Rong Hua Jianyu Tian Xin Wang Zuomin Zhou Jie Hao Jinjin Yu Dong Hua Jiayin Liu Xuejiang Guo Qi Zhou Jiahao Sha 
supported by National Key R&D Program(2017YFA0103803);National Natural Science Foundation of China(31890784);National Key R&D Program(2016YFA0500903,2016YFA0503300).
Unlike most organs that mature during the fetal period,the male reproductive system reaches maturity only at puberty with the commencement of spermatogenesis.Robust modelling of human testicular organogenesis in vitro...
关键词：FETAL ORGANS fertility 
A two-step lineage reprogramming strategy to generate functionally competent human hepatocytes from fibroblasts被引量：10
《Cell Research》2019年第9期696-710,共15页Bingqing Xie Da Sun Yuanyuan Du Jun Jia Shicheng Sun Jun Xu Yifang Liu Chengang Xiang Sitong Chen Huangfan Xie Qiming Wang Guangya Li Xuehui LYU Hui Shen Shiyu Li Min Wu Xiaonan Zhang Yue Pu Kuanhui Xiang Weifeng Lai Peng Du Zhenghong Yuan Cheng Li Yan Shi Shichun Lu Hongkui Deng 
We thank Prof. Tungtien Sun for the guidance of the written manuscript. This work was supported by National Key Research and Development Program of China (2017YFA0104003,2017YFA0103000,2018YFA0108102);National Natural Science Foundation of China(31730059,31521004,81670590);SLS-Qidong Innovation Fund(2016000663);National Basic Research Program of China(973 Program 2015CB964703).
Terminally differentiated cells can be generated by lineage reprogramming,which is,however,hindered by incomplete conversion with residual initial cell identity and partial functionality. Here, we demonstrate a new re...
关键词：hepatic progenitor-like cells (hHPLCs) primary human HEPATOCYTES (PHHs) FIBROBLASTS 
Persistent hepatitis C virus infections and hepatopathological manifestations in immune-competent humanized mice被引量：7
《Cell Research》2014年第9期1050-1066,共17页Jizheng Chen Yang Zhao Chao Zhang Hairong Chen Jin Feng Xiumei Chi Yu Pan Jun Du Min Guo Huang Cao Honghe Chen Zilong Wang Rongjuan Pei Qian Wang Lei Pan Junqi Niu Xinwen Chen Hong Tang 
Acknowledgments We are grateful to Drs T Wakita （National Institute of Infec- tious Diseases, Japan） for pJFH-1 clone, F Chisari （The Scripps Research Institute） for Huh7.5.1 cell line, L Su （University of North Carolina） for cDNA clones of human CD81 and Occludin, and C Rice （The Rockefeller University） for NS5A antibody. We thank Drs Y Tian and S Meng for making a series of transgenic mice; C-X Wei for the genotyping of transgenic mice. We thank Drs L Su, Y-X Fu （The University of Chicago）, R Sun （University of California, Los Angeles） and P Hertzog （Monash University, Australia） for their critical reading and comments on the manuscript. This work was supported by grants from the National Basic Research Program of China （2009CB522506 and 2011CB946104 to HT, 2009CBl18903 to XC, and 2010CB530100 to LP）, the National Natural Science Foundation of China （31200135 to XC and 31030031 to HT）, Knowledge Innovation Program Projects of Chinese Academy of Science （2010-Biols-CAS-0201to HT, and KSCXI-YW-10 to XC and HT）.
The majority of hepatitis C virus （HCV） infection develops chronic infection, which causes steatosis, cirrhosis and hepatocellular carcinoma. However, understanding HCV chronicity and pathogenesis is hampered by its...
关键词：HCV animal model humanized mice immune-competent 
Mice generated from tetraploid complementation competent iPS cells show similar developmental features as those from ES cells but are prone to tumorigenesis被引量：11
《Cell Research》2011年第11期1634-1637,共4页ManTong  ZhuoLv  LeiLiu  HuiZhu  Qin-YuanZheng  Xiao-YangZhao  WeiLi  Yi-BoWu  Hai-JiangZhang  Hua-JunWu  Zhi-KunLi  FanyiZeng  LiuWang  Xiu-JieWang  Jia-HaoSha QiZhou 
Dear Editor, Ever since the creation of induced pluripotent cells (iPSCs) from adult somatic cells by the ectopic expres- sion of defined transcription factors [1, 2], whether iPS cells are equivalent to embryonic st...
关键词：胚胎干细胞 iPS 四倍体 相似 小鼠 主管 互补 肿瘤 
Activation of paternally expressed imprinted genes in newly derived germline-competent mouse parthenogenetic embryonic stem cell lines被引量：10
《Cell Research》2007年第9期792-803,共12页Hua Jiang Bowen Sun Weicheng Wang Zhihong Zhang Furong Gao Guilai Shi Bing Cui XiangyinKong Zhao He Xiaoyan Ding Ying Kuang Jian Fei Yi Juan Sun Yun Feng Ying Jin 
Parthenogenetic embryonic stem （pES） cells provide a valuable in vitro model system for studying the molecular mechanisms that underlie genomic imprinting. However, the pluripotency of pES cells and the expression p...
关键词：PARTHENOGENESIS embryonic stem cell PLURIPOTENCY imprinted gene methylation 
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