ENZYMOLOGY

作品数：28被引量：89H指数：5
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Ergothioneine and its congeners:anti-ageing mechanisms and pharmacophore被引量：4
《Protein & Cell》2024年第3期191-206,共16页Li Chen Liping Zhang Xujun Ye Zixin Deng Changming Zhao 
supported by grants from the National Key R&D Program of China(No.2018YFA0903200 to C.Z.);the National Natural Science Foundation of China(Nos.32270032 and 32070038 to C.Z.,No.32000029 to L.C.).
Ergothioneine,Ovothiol,and Selenoneine are sulfur/selenium-containing histidine-derived natural products widely distributed across different organisms.They exhibit significant antioxidant properties,making them as pot...
关键词：ERGOTHIONEINE antioxidant ANTI-AGEING LONGEVITY biosynthesis ENZYMOLOGY 
Characteristics of Mycobacterium tuberculosis serine protease Rv1043c in enzymology and pathogenicity in mice
《Journal of Integrative Agriculture》2023年第12期3755-3768,共14页TANG Yang-yang CUI Ying-ying JIANG Yan-yan SHAO Ming-zhu ZANG Xin-xin DANG Guang-hui LIU Si-guo 
This research was supported by the National Key Research and Development Program of China(2021YFD1800403);the National Natural Science Foundation of China(32273005 and 32002256).
The serine proteases of Mycobacteria tuberculosis(Mtb)are important contributors to the process of bacterial invasion and its pathogenesis.In the present study,we systematically characterized the role of the Rv1043c p...
关键词：Mycobacterium tuberculosis Mycobacterium smegmatis serine protease Rv1043c PATHOGENICITY 
Electrophoretic Purification and Characterization of Human NADH-Glutamate Dehydrogenase Redox Cycle Isoenzymes Synthesizing Nongenetic Code-Based RNA Enzyme
《Advances in Enzyme Research》2021年第2期19-35,共17页Godson O. Osuji Wenceslaus C. Madu Paul M. Johnson 
NADH-glutamate dehydrogenase (GDH) is active in human tissues, and is chromatographically purified, and studied because it participates in synthesizing glutamate, a neurotransmitter. But chromatography dissociates the...
关键词：GDH Electrophoretic Enzymology Chromatographic GDH Total RNA-RNA Enzyme Complex mRNA Cohorts Chemical Dependency 
Purification and Characterization of a Novel Lipase from Antarctic Krill被引量：2
《Journal of Ocean University of China》2020年第1期209-215,共7页CHEN Xin WANG Chunlan XU Jiakun WANG Fang JIANG Yihui CHEN Yixuan and ZHAO Xianyong 
supported by the Marine S&T Fund of Shandong Province for the Pilot National Laboratory for Marine Science and Technology (Qingdao) (Nos. 2018 SDKJ0304-4-2, 2018SDKJ0303-1);the Central Public-Interest Scientific Institution Basal Research Fund, Chinese Academy of Fishery Sciences (Nos. 2017HY-XKQ01-01, 2016ZD0902, 2018GH10);the Central Public-Interest Scientific Institution Basal Research Fund, YSFRI, CAFS (No. 20603022018025);the Aoshan S&T Innovation Project from Qingdao National Laboratory for Marine Science and Technology (No. 2015ASKJ02-02-04);the Antarctic Marine Biological Resources Development and Utilization Project from the Ministry of Agriculture and Rural Affairs, People’s Republic of China (2017);the Financial Fund of the Ministry of Agriculture and Rural Affairs, People’s Republic of China (Nos. NFZX2018, FSTICE2019)
Lipase from Antarctic krill,with a molecular weight of 71.27kDa,was purified with ammonium sulfate precipitation and a series of chromatographic separations over ion exchange(DEAE)and gel filtration columns(Sephacryl ...
关键词：Antarctic krill LIPASE isolation and purification enzymology properties 
Inactivity of YGL082W in vitro due to impairment of conformational change in the catalytic center loop被引量：1
《Science China Chemistry》2020年第2期237-243,共7页Lining Lu Yu Guo Tian Wang Lujun Liang Suwen Zhao Feng Wang Lei Liu 
supported by the National Key Research and Development Program of China(2017YFA0505200);the National Natural Science Foundation of China(21532004,91753205,81621002,21621003);Shanghai Tech University
MINDY-1 is a recently discovered new family of deubiquitinating enzymes(DUB),but one of its yeast homologs,YGL082 W,does not show any DUB activity in vitro.Sequence alignment shows that YGL082 W possesses the correct ...
关键词：deubiquitinating enzymes(DUB) ENZYMOLOGY X-ray crystal structure molecular dynamics 
Nanozymes: an emerging field bridging nanotechnology and enzymology被引量：11
《Science China(Life Sciences)》2019年第11期1543-1546,共4页Xiangqin Meng Kelong Fan Xiyun Yan 
financially supported by the Strategic Priority Research Program(XDB29040101);the National Natural Science Foundation of China(31900981,31871005,31530026);Chinese Academy of Sciences(YJKYYQ20180048);the Key Research Program of Frontier Sciences(QYZDY-SSW-SMC013);Chinese Academy of Sciences and National Key Research and Development Program of China(2017YFA0205200);Youth Innovation Promotion Association CAS(2019093)
Nanozyme,a class of nanomaterials with intrinsic enzymelike properties,is a new concept which has been included in the Encyclopedia of China and the textbook of enzyme engineering.Since the first evidence published in...
关键词：BRIDGING FIELD INTRINSIC 
A versatile platform for single-molecule enzymology of restriction endonuclease
《Journal of Innovative Optical Health Sciences》2019年第1期29-38,共10页Xin Wang Jingyuan Nie Yi Li Hai Pan Peng Zheng Meng Qin Yi Cao Wei Wang 
The authors greatly appreciate the-nancial support from National Natural Science Foundation of China(Grant Nos.21522402,11674153,11374148,11334004 and 21771103);the Fundamental Research Funds for the Central Universities(Nos.020414380070,020414380050 and 020414380058);Natural Science Foundation of Jiangsu Province(No.BK20160639)and the Shuangchuang Program of Jiangsu Province.
Enzymes are the major players for many biological processes.Fundamental studies of the enzymatic activity at the single-molecule level provides important information that is otherwise inaccessible at the ensemble leve...
关键词：Single molecule Forster resonance energy transfer ENZYMOLOGY KINETICS holliday junction 
Biosynthesis and molecular engineering of templated natural products被引量：4
《National Science Review》2017年第4期553-575,共23页Ming Chen Jingyu Liu Panpan Duan Mulin Li Wen Liu 
supported in part by grants from National Natural Science Foundation of China(21520102004,31430005,21472231 and 21621002);Science and Technology Commission of Shanghai Municipality(Shanghai,China)(14JC1407700 and15JC1400400);Chinese Academy of Sciences(XDB20020200);Chang-Jiang Scholars Program of China
Bioactive small molecules that are produced by living organisms, often referred to as natural products(NPs), historically play a critical role in the context of both medicinal chemistry and chemical biology. How natur...
关键词：natural products BIOSYNTHESIS template enzymology 
Enzymology properties of two different xylanases and their impacts on growth performance and intestinal microflora of weaned piglets被引量：3
《Animal Nutrition》2016年第1期18-23,共6页Qinghua Chen Mengwei Li Xiong Wang 
supported by the 'the twelfth five-year-plan' of China national science and technology for the rural development (2013BAD10B00)
The enzyme xylanase is more and more widely used in feed production, but different xylanase have different properties, mechanism and application effects. To provide a theoretical basis for choosing more suitable xylan...
关键词：XYLANASE Enzymology characteristics Weaned piglet Growth performance Microbial flora 
Complex Regulation of Prolyl-4-Hydroxylases Impacts Root Hair Expansion
《Molecular Plant》2015年第5期734-746,共13页Silvia M. Velasauez Martiniano M. Ricardi Christian Peter Poulsen Ai Oikawa Adiphol Dilokpimol Adnan Halim Silvina Mangano Silvina Paola Denita Juarez Eliana Marzol Juan D. Salgado Salter Javier Gloazzo Dorosz Cecilia Borassi Svenning Rune Moller Rafael Buono Yukiko Ohsawa Ken Matsuoka Marisa S. Otegui Henrik V. Scheller Naomi Geshi Bent Larsen Petersen Norberto D. lusem Jose M. Estevez 
Root hairs are single cells that develop by tip growth, a process shared with pollen tubes, axons, and fungal hyphae. However, structural plant cell walls impose constraints to accomplish tip growth. In addition to po...
关键词：ENZYMOLOGY cell expansion cell walls protein targeting proline hydroxylation root hairs 
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