POLYHEDRIN

作品数：8被引量：2H指数：1
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相关领域：生物学更多>>
相关作者：安成才林同张传溪刘宽余王志英更多>>
相关机构：东北林业大学北京大学浙江大学中国热带农业科学院更多>>
相关期刊：《BioDesign Research》《Wuhan University Journal of Natural Sciences》《Journal of Biomedical Science and Engineering》《Science Bulletin》更多>>
相关基金：国家自然科学基金国家科技支撑计划更多>>
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Phagocytosed Polyhedrin-Cytokine Cocrystal Nanoparticles Provide Sustained Secretion of Bioactive Cytokines from Macrophages
《BioDesign Research》2021年第1期47-58,共12页Astrid Wendler Nicholas James Michael H.Jones Christian Pernstich 
Many cells possess the ability to engulf and incorporate particles by phagocytosis.This active process is characteristic of microorganisms as well as higher order species.In mammals,monocytes,macrophages,and microglia...
关键词：SUSTAINED HEALING professional 
Analysis and expression of the polyhedrin gene of Antheraea pernyi nucleopolyhedrovirus (AnpeNPV)
《Journal of Biomedical Science and Engineering》2009年第2期128-134,共7页Jia-Xi Huang Hui-Ling Wu Yan Wu Shan-Ying Zhu Wen-Bing Wang 
The polyhedrin (polh) gene is often used to analyse evolution of baculovirus. In this report, the polh of Antheraea pernyi nucleopolyhedro-virus (AnpeNPV) was cloned and sequenced. The Open reading frame (ORF) of the ...
关键词：Antheraea Pernyi Insect BACULOVIRUS NPV POLYHEDRIN PROKARYOTIC EXPRESSION 
AcNPV polyhedrin与cry3Aa基因的重组与重组融合蛋白在大肠菌中的表达
《中国生物工程杂志》2008年第S1期65-69,共5页高博 邓柳红 易小平 张春发 
国家科技支撑计划资助项目(2007BAD48B02-3)
目的:将cry3Aa基因与斜纹夜蛾多角体蛋白(AcNPV Polyhedrin)基因重组,构建重组蛋白原核表达载体并在大肠杆菌中表达。方法:首先以PHT305a载体质粒DNA为模板,PCR扩增cry3Aa基因。再以PET30a-ph-1a载体质粒DNA为模板,PCR扩增多角体蛋白基...
关键词：BT cry3Aa ACNPV POLYHEDRIN 重组 表达 
THE CLONING, SEQUENCING AND EXPRESSION OF THE LdMNPV POLYHEDRIN GENE被引量：2
《Entomologia Sinica》2002年第4期47-52,共6页林同 张传溪 安成才 王志英 刘宽余 苑华毅 
国家自然科学基金项目(30070032);高等学校全国优秀博士学位论文作者专项基金(200055)
LdMNPV-NEFU isolate collected from the forestry farm of Northeast Forestry University was purified and the genomic DNA of LdMNPV was extracted. The LdMNPV polyhedrin gene was cloned by PCR. The results showed that the...
关键词：LdMNPV polyhedrin gene CLONE sequence EXPRESSION 
Mutagenic Analysis on the Polyhedrin gene(polh) of Bombyx mori Nuclear Polyhedrosis virus(BmNPV)
《癌变．畸变．突变》2001年第4期241-241,共1页Cao Yang Huang Ya－dong 等 
关键词：家蚕 核多角体病毒 polh基因突变 遗传分析 
Expression　of　Green　Fluorescent　Protein　Gene　with　Baculovirus　Vectorin　Insect　Cells
《Wuhan University Journal of Natural Sciences》1997年第1期117-121,共5页Hu Jianhong Zhu Fanxiu Qi Yipeng Huang Yongxiu 
The green fluorescence of bioluminescent jellyfish Aequorea victoria is due to the presence of the green fluorescent protein (GFP). To examine whether the GFP gene can be applied as a reporter gene in insect cells...
关键词：green fluorescent protein(GFP) BACULOVIRUS transfer vector insect cells polyhedrin gene neomycin resistance gene 
Construction of polyhedrin-positive recombinant viruswith expression of truncated δ-endotoxin fromBacillus thuringiensis in insect cell
《Chinese Science Bulletin》1996年第7期597-603,共7页王福山 黄永秀 齐义鹏 刘子夜 杨远征 
Baculoviruses have been widely used as biological agents because of their specificpathogenicity for target insect and harmlessness to mammals, birds and plants as well asadvantages with persistence and epidemics as pe...
关键词：Bacillus THURINGIENSIS δ-endotoxin POLYHEDRIN gene EXPRESSION recombinant virus. 
Nucleotide　Sequence　of　Polyhedrin　Gene　of　LsNPV　and　Analysis　of　Baculovirus　Polyhedron　Proteins
《Wuhan University Journal of Natural Sciences》1996年第2期272-278,共7页Wang Jiawang Qi Yipeng Deng Yanhui Mallam Nock Joshua 
The intact 741 hp polyhedrin gene of LsNPV was sequenced by Silver Sequencing System, and shares 90.6% and 97.0% nucleotide identity, 97.2% and 97. 6% amino acid identity with PfNPV and MdNPV polh genes respectively....
关键词：LsNPV BACULOVIRUS polyhedrin gene SEQUENCE HOMOLOGY 
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