AMINOACYLASE

作品数：24被引量：6H指数：1
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相关领域：生物学理学更多>>
相关作者：李莉莉李晓敏宋正孝李牧彭英更多>>
相关机构：长春理工大学天津大学更多>>
相关期刊：《Chemical Research in Chinese Universities》《Chinese Journal of Reactive Polymers》《Acta Pharmacologica Sinica》《Science China Chemistry》更多>>
相关基金：国家自然科学基金天津市自然科学基金中国博士后科学基金浙江省自然科学基金更多>>
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Aminoacylase from pig kidney contains no disulfide bonds
《Science China Chemistry》1995年第12期1448-1454,共7页王洪睿 张彤 王泽峰 王希成 周海梦 
Both non-reduced/reduced(NR/R)two-dimensional diagonal SDS-PAGE and NR/Rone-dimensional SDS-PAGE showed no disulfide bonds in aminoacylase from pig kidney.Eight andfour thiol groups were modified in the native enzyme ...
关键词：AMINOACYLASE DISULFIDE bonds two-dimensional DIAGONAL SDS-PAGE CYSTEINE THIOL group. 
Comparison between conformational change and inactivation rates of aminoacylase during denaturation in urea solutions
《Science China Chemistry》1995年第3期328-335,共8页王洪睿 王希成 张彤 周海梦 
The kinetic method of the substrate reaction in the presence of mactivator previously described by Tsou has been applied to the determination of inactivation rates of aminoacylase during denaturation in urea solutions...
关键词：AMINOACYLASE DENATURATION INACTIVATION CONFORMATION kinetics. 
Secondary Structure of Holo-Enzyme and Apo-Enzyme of Aminoacylase Using CD and FTIR Spectroscopy
《Science China Chemistry》1994年第12期1471-1478,共8页张艳 陈培榕 何飚 周海梦 
Aminoacylase is a dimeric metal enzyme containing one Zn^(2+)-ion per subunit of active site. It is essential for the activity of enzyme.Fourier transform-infrared spectroscopy has been used for the study on the secon...
关键词：AMINOACYLASE FTIR SPECTRUM SECONDARY structure. 
Studies on Immobilization of Aminoacylase From Aspergillus Oryzae on Macroporous Carriers
《Science China Chemistry》1993年第7期778-784,共7页李民勤 王道宾 周菊岩 何炳林 
<正> Synthesized macroporous cross-linked copolymers of methyl acrylate-divinyl benzene (MA-DVB), acrylamide-N,N’-methylenebisacrylamide (AAM-BIS) and their functionalized products were used for immobilization of ami...
关键词：eross-linked COPOLYMER of methyl acrylate-divinyl benzene cross-linked COPOLYMER of acrylamide-N N’-methylenebisacrylamide aminoacy lase from Aspergillus ORYZAE immobilied enzyme. 
Kinetics of Inactivation of Aminoacylase I During Modification of Its Thiol Groups by DPDS and PCMB被引量：2
《Science China Chemistry》1993年第1期52-59,共8页王洪睿 阴勇 张英侠 王希成 周海梦 
The kinetics theory of the substrate reaction during modification of enzyme activity previously described by Tsou has been applied to a study on the kinetics of the course of inactivation of aminoacylase I by DPDS and...
关键词：AMINOACYLASE CHEMICAL MODIFICATION INHIBITION kinetics. 
THE IMMOBILIZATION OF AMINOACYLASE AND THE RESOLUTION OF D,L-PHENYLALANINE被引量：1
《Science China Chemistry》1991年第6期641-650,共10页韩际宏 姜坪 何炳林 
<正> A polymer with suitable physical characters and matched functional groups was synthesized,and used as a supporter to immobilize aminoacylase. The results showed that this supporterhad high immobilizing capacity a...
关键词：AMINOACYLASE IMMOBILIZATION PHENYLALANINE RACEMATE RESOLUTION 
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