AMINOACYLASE

作品数：24被引量：6H指数：1
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相关领域：生物学理学更多>>
相关作者：李莉莉李晓敏宋正孝李牧彭英更多>>
相关机构：长春理工大学天津大学更多>>
相关期刊：《Chemical Research in Chinese Universities》《Chinese Journal of Reactive Polymers》《Acta Pharmacologica Sinica》《Science China Chemistry》更多>>
相关基金：国家自然科学基金天津市自然科学基金中国博士后科学基金浙江省自然科学基金更多>>
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Improved Method for Lacosamide Synthesis with Chemoenzymatic Method
《Chemical Research in Chinese Universities》2012年第5期833-836,共4页WANG Zhi-yuan XU Li-sheng LIU Jun-zhong ZHANG Hong-juan GAO Ji LIU Qian JIAO Qing-cai 
Supported by the National Technology-Innovation Fund(No.02CJ-13-01-16);the Open Fund of State Key Laboratory of Pharmaceutical Biotechnology of Nanjing University, China
Lacosamide was prepared by chemical method coupled with enzymatic method. N-Acetyl-D, L-3-methoxy-alanine, derived from D,L-3-methoxy-alanine, was used in the resolution process catalyzed by immobilized Escherichia co...
关键词：LACOSAMIDE AMINOACYLASE N-Acetyl-D L-3-methoxy-alanine Immobilized cell 
Immobilization of Aspergillus Oryzae Mycelium Pellets and Its Application in the Resolution of D, L-Alanine
《Transactions of Tianjin University》2006年第4期242-247,共6页王燕 朱怀工 张凤宝 王淑兰 蔡旺锋 张旭斌 
Aspergillus oryzae 3042 mycelium pellets were immobilized by crosslinking method with reagents of gelatin and formaldehyde. An orthogonal design table was used to determine the optimal immobilization conditions. The L...
关键词：L-aminoacylase Aspergillus oryzae IMMOBILIZATION CROSSLINKING ALANINE pHi temperature 
Low Temperature Induced Conformation Changes of Aminoacylase
《Tsinghua Science and Technology》2004年第1期76-80,共5页谢强 孟凡国 周海梦 
the National Key Basic Research and Development (973) Program of China (No. G1999075607)
Control of aggregation, by lowering temperature and protein concentrations, can enhance the extent of successful refolding. The low temperature has been used in protein folding studies, as undesired aggregations often...
关键词：low temperature AMINOACYLASE secondary structure 
Enhancement of Aminoacylase Activity by Sodium Citrate
《Tsinghua Science and Technology》2001年第5期421-425,共5页于范利 曹志方 李森 周海梦 
Kidney and other tissues of animals and humans have a high concentration of citrate which is an important intermediate substance in the citrate cycle. Citrate may play an important physiological role in metabolism. ...
关键词：AMINOACYLASE sodium citrate ACTIVATION DENATURATION UNFOLDING 
IMMOBILIZED ASPERGILLUS ORYZAE CELLS AND THEIR ACTIVITIES
《Transactions of Tianjin University》1997年第2期14-18,共5页宋正孝 李晓敏 王诤 彭英 
Aminoacylase was immobilized on the mycelium cells of Aspergillus oryzae by using ethylenediamine, gelatin and glutylalhyde. The proper immobilized condition was studied by orthogonal experimental design. The immo...
关键词：IMMOBILIZATION ASPERGILLUS AMINOACYLASE optically resolution 
Aminoacylase from pig kidney contains no disulfide bonds
《Science China Chemistry》1995年第12期1448-1454,共7页王洪睿 张彤 王泽峰 王希成 周海梦 
Both non-reduced/reduced(NR/R)two-dimensional diagonal SDS-PAGE and NR/Rone-dimensional SDS-PAGE showed no disulfide bonds in aminoacylase from pig kidney.Eight andfour thiol groups were modified in the native enzyme ...
关键词：AMINOACYLASE DISULFIDE bonds two-dimensional DIAGONAL SDS-PAGE CYSTEINE THIOL group. 
Inactivation of aminoacylase at low urea concentrations not due to dissociation of dimeric enzyme
《Chinese Science Bulletin》1995年第6期517-521,共5页王希成 张彤 王洪睿 周海梦 
Aminoacylase （EC 3. 5. 1. 14） is a dimeric enzyme containing one Zn2+ ion per subunit with a molecular weight of 43000 u. Zn2+ ion is located at the active site, and it is essential for enzyme ...
关键词：AMINOACYLASE DISSOCIATION urea-gradient ELECTROPHORESIS unfolding. 
Unfolding and Inactivation During Thermal Denaturation of Aminoacylase
《Chinese Science Bulletin》1994年第13期1122-1127,共6页何飚 严明 张彤 周海梦 
Many researchers have compared inactivation with conformational changes of a number of enzymes during denaturation by guanidine hydrochloride and urea. The results obtained show that inactivation occurs before noticea...
关键词：AMINOACYLASE THERMAL DENATURATION INACTIVATION unfolding. 
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