PICHIA_PASTORIS

作品数：195被引量：497H指数：10
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Screening and characterization of integration sites based on CRISPR-Cpf1 in Pichia pastoris
《Synthetic and Systems Biotechnology》2024年第4期759-765,共7页Shupeng Ruan Yuxin Yang Xinying Zhang Guanjuan Luo Ying Lin Shuli Liang 
supported by the National Key Research and Development Program(2021YFC2104000);the National Natural Science Foundation of China(32272276);the Fundamental Research Funds for the Central Universities.
Pichia pastoris,a methylotrophic yeast,can utilize methanol as a carbon source and energy source to synthesize high-value chemicals,and is an ideal host for biomanufacturing.Constructing the P.pastoris cell factory is...
关键词：P.PASTORIS CRISPR-Cpf1 Integration sites Metabolic engineering 
Combined strategies for improving the heterologous expression of a novel xylanase from Fusarium oxysporum Fo47 in Pichia pastoris
《Synthetic and Systems Biotechnology》2024年第3期426-435,共10页Chun Liu Yaping Zhang Chunting Ye Fengguang Zhao Yian Chen Shuangyan Han 
supported by the Key-Area Research and Development Program of Guangdong Province(2020B020226007);National Key Research and Development Program of China(2021YFC2100405,2022YFC2105501).
Xylanase,an enzyme capable of hydrolyzing non-starch polysaccharides found in grain structures like wheat,has been found to improve the organizational structure of dough and thus increase its volume.In our past work,o...
关键词：XYLANASE Pichia pastoris Heterologous protein Secretion expression 
Expression and antimicrobial activity of the recombinant bovine lactoferricin in Pichia pastoris
《Synthetic and Systems Biotechnology》2024年第1期26-32,共7页Xueqin Lv Yuting Zhang Lingrui Wang Shixiu Cui Yanfeng Liu Jianghua Li Guocheng Du Long Liu 
supported by the National Natural Science Foundation of China(32021005,32270096);the Fundamental Research Funds for the Central Universities(JUSRP222007,JUSRP622004).
Lactoferricin,a multifunctional peptide located in the N-terminal region of lactoferrin,has a broad-spectrum bacteriostatic activity.It is a promising candidate as a food additive and immune fortification agent and do...
关键词：Antimicrobial peptide LACTOFERRICIN Recombinant expression Pichia pastoris 
Methanol-based biomanufacturing of fuels and chemicals using native and synthetic methylotrophs被引量：5
《Synthetic and Systems Biotechnology》2023年第3期396-415,共20页Arslan Sarwar Eun Yeol Lee 
the C1 Gas Refinery Program through the National Research Foundation of Korea(NRF),funded by the Ministry of Science and ICT(2015M3D3A1A01064882);the Korea Institute of Marine Science&Technology Promotion(KIMST),funded by the Ministry of Oceans and Fisheries,Korea(20220532).
Methanol has recently gained significant attention as a potential carbon substrate for the production of fuels and chemicals,owing to its high degree of reduction,abundance,and low price.Native methylotrophic yeasts a...
关键词：Native methylotrophs Synthetic methylotrophs Pichia pastoris Methylobacterium extorquens Bacillus methanolicus Escherichia coli 
A novel CRISPR/Cas9 system with high genomic editing efficiency and recyclable auxotrophic selective marker for multiple-step metabolic rewriting in Pichia pastoris被引量：2
《Synthetic and Systems Biotechnology》2023年第3期445-451,共7页Xiang Wang Yi Li Zhehao Jin Xiangjian Liu Xiang Gao Shuyuan Guo Tao Yu 
Key-Area Research and Development Program of Guangdong Province(2022B1111080005);the National Key Research and Development Program of China(2020YFA0907800 and 2021YFA0911000);the National Natural Science Foundation of China(NSFC 32071416);the Shenzhen Institute of Synthetic Biology Scientific Research Program(Grant No.JCHZ20200003);Shenzhen Key Laboratory for the Intelligent Microbial Manufacturing of Medicines(ZDSYS20210623091810032);the Strategic Priority Research Program of the Chinese Academy of Sciences(XDB0480000);the China Postdoctoral Science Foundation(2020M682973);Guangdong Basic and Applied Basic Research Foundation(2020A1515110927).
The methylotrophic budding yeast Pichia pastoris has been utilized to the production of a variety of heterologous recombinant proteins owing to the strong inducible alcohol oxidase promoter(pAOX1).However,it is diffic...
关键词：Pichia pastoris Uracil auxotroph CRISPR/Cas9 Recyclable selective marker Homology directed repair 
An improved CRISPRi system in Pichia pastoris被引量：2
《Synthetic and Systems Biotechnology》2023年第3期479-485,共7页Shujing Qiao Fan Bai Peng Cai Yongjin J.Zhou Lun Yao 
National Key Research and Development Program of China(2021YFC2103500);Dalian Institute of Chemical Physics Innovation Program(DICP I202111).
CRISPR interference(CRISPRi)has been developed and widely used for gene repression in various hosts.Here we report an improved CRISPRi system in Pichia pastoris by fusing dCas9 with endogenous transcriptional represso...
关键词：Pichia pastoris CRISPRi HH/HDV Csy4 gRNA array 
De novo biosynthesis of a vinblastine precursor in Pichia pastoris
《Synthetic and Systems Biotechnology》2023年第2期300-301,共2页Jiaming Yu Dongyuan Lv Lixin Zhang 
Plant natural products have been widely used in medicine,food,and cosmetics.Of a particular note,many natural products from Cathar-anthus roseus have important biological activities and medicinal value^([1]),with vinb...
关键词：products. synthesis PREPARATION 
Comparative proteomics analysis of Pichia pastoris cultivating in glucose and methanol被引量：2
《Synthetic and Systems Biotechnology》2022年第3期862-868,共7页Rui Hou Linhui Gao Jianhui Liu Zhen Liang Yongjin J.Zhou Lihua Zhang Yukui Zhang 
supported by National Natural Science Foundation of China(22161142008,M-0246);DMTO research grant(grant no.DICP DMTO_(2)01701)from Dalian institute of Chemical Physics,CAS.
The methylotrophic yeast Pichia pastoris(syn.Komagataella phaffii)has been extensively engineered for protein production,and is attracting attention as a chassis cell for methanol biotransformation toward production o...
关键词：PROTEOMICS PEROXISOME Methanol metabolism Systems biology 
Development of synthetic biology tools to engineer Pichia pastoris as a chassis for the production of natural products被引量：16
《Synthetic and Systems Biotechnology》2021年第2期110-119,共10页Jucan Gao Lihong Jiang Jiazhang Lian 
supported by the National Key Research and Development Program of China(2018YFA0901800);the Natural Science Foundation of China(21808199);the Natural Science Foundation of Zhejiang Province(LR20B060003).
The methylotrophic yeast Pichia pastoris(a.k.a.Komagataella phaffii)is one of the most commonly used hosts for industrial production of recombinant proteins.As a non-conventional yeast,P.pastoris has unique biological...
关键词：Pichia pastoris Natural products Synthetic biology CRISPR/Cas9 Heterologous gene expression 
Improving the reaction mix of a Pichia pastoris cell-free system using a design of experiments approach to minimise experimental effort被引量：1
《Synthetic and Systems Biotechnology》2020年第3期137-144,共8页Alex J.Spice Rochelle Aw Daniel G.Bracewell Karen M.Polizzi 
This research is funded by the Department of Health and Social Care using UK Aid funding and is managed by the Engineering and Physical Sciences Research Council(EPSRC,grant number:EP/R013764/1).
A renaissance in cell-free protein synthesis(CFPS)is underway,enabled by the acceleration and adoption of synthetic biology methods.CFPS has emerged as a powerful platform technology for synthetic gene network design,...
关键词：Cell-free protein synthesis Pichia pastoris Synthetic biology Design of experiments(DOE) 
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