SEPHAROSE

作品数:93被引量:223H指数:8
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相关领域:生物学更多>>
相关作者:毛琦琦赵丹叶强李茂光李亚南更多>>
相关机构:暨南大学四川大学吉林大学中国食品药品检定研究院更多>>
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Enhanced production of cytidine 5'-monophosphate using biocatalysis of di-enzymes immobilized on amino-functionalized sepharose
《Chinese Journal of Chemical Engineering》2023年第6期40-52,共13页Xiaohong Zhou Wenfeng Zhou Wei Zhuang Chenjie Zhu Hanjie Ying Hongman Zhang 
supported by grants from the National Key Research and Development Program of China(2021YFC2102805,2019YFD1101204);the National Natural Science Foundation of China(21878142,21776132);Key Research and Development Plan of Jiangsu Province(BE2020712);Key Research and Development Plan of Jiangsu Province(BE2019001);Jiangsu Natural Science Fund for Distinguished Young Scholars(BK20190035);the Priority Academic Program Development of Jiangsu Higher Education Institutions(PAPD)。
Cytidine 5'-monophosphate(5'-CMP)is an essential nucleotide for additives.In this study,enhanced production of 5'-CMP was realized by the transformation of cytidine using co-immobilized di-enzymes,uridine-cytidine kin...
关键词:SEPHAROSE Ε-POLYLYSINE Dual-enzyme cascade Cytidine 5'-monophosphate Enzyme immobilization 
Lysozyme adsorption to cation exchanger derivatized by sequential modification of poly(ethylenimine)-Sepharose with succinic anhydride and ethanolamine: Effect of p H and ionic strength被引量:2
《Chinese Journal of Chemical Engineering》2020年第2期440-444,共5页Yangyang Zhao Xianxiu Li Linling Yu Xiaoyan Dong Yan Sun 
supported by the National Natural Science Foundation of China (Nos. 21878222 and 21621004).
In our previous work, a series of polyethylenimine(PEI)-derived cation exchangers were synthesized using PEIgrafted resin FF-PEI-L740(ionic capacity, 740 mmol·L^-1) as the basic resin to study lysozyme adsorption and...
关键词:Ion exchange pH value Adsorption Kinetics CHAIN delivery Dynamic BINDING capacity 
Implications from protein uptake kinetics onto dextran-grafted Sepharose FF coupled with ion exchange and affinity ligands被引量:10
《Chinese Journal of Chemical Engineering》2017年第7期906-910,共5页Aiying Xue Linling Yu Yan Sun 
Supported by the National Natural Science Foundation of China(21236005,21621004)
Our previous studies have reported the presence of "chain delivery" effects of protein adsorption onto ion exchangers with polymer-grafted ion-exchange groups, such as dextran-grafted and poly(ethylenimine)-modified S...
关键词:Dextran-grafted adsorbent Ion exchange chromatography Affinity chromatography lmmunoglobulin G KINETICS 
大豆巯基肽的共价色谱法制备及其MALDI-TOF-MS分析被引量:2
《中国油脂》2016年第12期31-36,共6页丁秀臻 孔祥珍 陈业明 张彩猛 华欲飞 
国家自然科学基金(31201380);江苏省自然科学基金(BK2011151)
建立了一种从复杂的大豆球蛋白酶解物中提取巯基肽的高选择性方法。首先,对大豆球蛋白酶解物中的二硫键用1,4-二硫苏糖醇进行还原,对还原剂的用量进行了考察和优化,提高了酶解物中的巯基肽含量。然后用Thiopropyl-Sephrose 6B共价色谱...
关键词:大豆球蛋白酶解物 巯基肽 还原 Thiolpropyl—Sepharose 6B MALDI—TOF—MS 
The separation single-wall carbon nanotubes on length by sepharose gel被引量:3
《Science China(Physics,Mechanics & Astronomy)》2012年第7期1178-1182,共5页REN LingLing ZHAO MingYuan WANG ZhiHua 
supported by the National Science & Technology Pillar Program of MOST (Grant No. 2011BAK15B04)
The separations of single-wall carbon nanotubes on length by sepharose gel were investigated in this work. The solutions of sodium dodecyl sulfate and sodium deoxycholate were applied as the eluent in sequence. SEM an...
关键词:single-wall carbon nanotubes LENGTH SEPARATION sepharose gel sodium dodecyl sulfate sodium deoxycholate 
Q Sepharose^(TM) XL强阴离子交换色谱快速纯化猪心中肌红蛋白和细胞色素C被引量:1
《化学与生物工程》2009年第10期56-59,共4页李琳子 徐朋朋 李任强 
暨南大学自然科学基金资助项目(640073)
以Q SepharoseTM XL强阴离子交换色谱结合Sephadex G-75分子排阻色谱快速提取纯化猪心中肌红蛋白(Myoglobin,Mb)和细胞色素C(Cyt C)。在pH值为7.6~8.5范围内,Cyt C吸附在Q SepharoseTMXL强阴离子交换柱上,而Mb会缓慢通过。所以选择QSeph...
关键词:蛋白纯化 Q SepharoseTM XL强阴离子交换色谱 肌红蛋白 细胞色素C 猪心 
用壳聚糖替代Sepharose 4B固定相关配体纯化酶的研究被引量:2
《工业微生物》2002年第1期19-22,共4页周毅彬 张培德 
采用壳聚糖固定酶作用的特定底物 (菌体细胞 ) ,并用戊二醛交联制备成酶的亲和吸附剂。采用该吸附剂纯化溶葡球菌酶的研究表明 ,经一步纯化可提高纯度 4倍 ,酶活性回收大于70 %。SDS PAGE的电泳结果显示 ,产品基本上达到了标准酶的纯度...
关键词:壳聚糖 固定化 金黄色葡萄球菌 溶葡球菌酶 溶菌酶 配体 
魔芋葡苷聚糖(KGM)凝胶为亲和层析载体与Sepharose 4B的比较研究 Ⅱ.KGM染料亲和层析分离人血清白蛋白被引量:3
《天然产物研究与开发》2000年第2期23-26,共4页周立 蒋磊 郑远旗 李建吾 
将 KGM和 Sepharose4 B凝胶在相同条件下活化、偶联接上染料 CibacronBlue F3GA制成了 KGM和 Sepharose 4 B染料亲和吸附剂 ,并用来与牛血清白蛋白( BSA)作用 ,每毫升 KGM亲和吸附剂可吸附 BSA2 8mg,用 Na SCN洗脱时回收为84 .5% ,而 Se...
关键词:魔芋葡苷聚糖 染料亲和层析 血清白蛋白 载体 
PREPARATION AND PROPERTIES OF HUMAN INSULIN被引量:1
《Chinese Science Bulletin》1985年第6期817-821,共5页朱尚权 崔大敷 
It is over 50 years since bovine and porcine insulins were used clinically to treat diabetes. Recently, human insulin prepared by genetic engineering and by enzymatic semisynthesis has become available. In this paper,...
关键词:PORCINE PREPARING ENZYMATIC BOVINE elsewhere SEPHAROSE clinically isolation FORMAMIDE subjee 
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