supported by the National Key Research and Development Program of China(2021YFC210500 and 2022YFC2104900);the National Natural Science Foundation of China(21961142016,22277111 and 22107094);Department of Science and Technology of Shandong Province(2020CXGC010601,2021ZDSYS22 and ZR2021QB061)。
An efficient enzymatic assembly strategy was developed for the concise synthesis of structurally well-defined sialylated chitooligosaccharides.Two enzyme modules for theβ-1,3-N-acetyl-glucosaminylation andβ-1,4-gala...