AGROBACTERIUM_TUMEFACIENS

作品数：87被引量：182H指数：7
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A simple,highly efficient Agrobacterium tumefaciensmediated moss transformation system with broad applications
《aBIOTECH》2024年第4期476-487,共12页Ping Zhou Xiujin Liu Yuqing Liang Yan Zhang Xiaoshuang Li Daoyuan Zhang 
supported by the Leading Talents in Technological Innovation programme(2022TSYCLJ0049);the Second Tibetan Plateau Scientific Expedition and Research(STEP)program(2019QZKK0502030403).
Mosses,particularly desiccation-tolerant(DT)species,are important model organisms for studying genes involved in plant development and stress resistance.The lack of a simple and efficient stable moss transformation sy...
关键词：Agrobacterium tumefaciens Highly efficient MOSS TRANSFORMATION 
等离子体活化水对根癌土壤杆菌的抑制作用及其对樱花根癌病的防治被引量：1
《北方园艺》2024年第9期54-61,共8页潘越 司红波 潘宇伟 孙永莲 
国家重点研发计划资助项目(2019YFD1002300);江苏高校哲学社会科学研究资助项目(2023SJYB0161)。
以樱花幼苗为试材,使用低温等离子体活化水冷杀菌技术(PAW)对樱花幼苗及其病原菌进行处理,研究了PAW对樱花苗木的抗病作用,以期为PAW在樱花根癌病防治中的应用提供参考依据。结果表明:等离子体活化水能有效地抑制樱花根癌病病菌Agrobact...
关键词：樱花 根癌病 Agrobacterium tumefaciens 等离子体活化水 
Enemies atpeace:Recentprogressin Agrobacterium-mediated cereal transformation
《The Crop Journal》2024年第2期321-329,共9页Shaoshuai Liu Ke Wang Shuaifeng Geng Moammar Hossain Xingguo Ye Aili Li Long Mao Karl-Heinz Kogel 
financial assistance provided by the High-End Foreign Expert Recruitment Program(G2022051003L);National Natural Science Foundation of China(32201878);Hainan Yazhou Bay Seed Lab(B21HJ0215);Agricultural Science and Technology Innovation Program of CAAS(CAASZDRW202002,CAAS-ZDRW202201);Hebei Natural Science Foundation(C2021205013);Long Mao is also a“Yellow River Delta Scholar”in Sino-Agro Experimental Station for Salt Tolerant Crops(SAESSTC),Dongying,Shandong,China.
Agrobacterium tumefaciens mediated plant transformation is a versatile tool for plant genetic engineering following its discovery nearly half a century ago.Numerous modifications were made in its application to increa...
关键词：Agrobacterium tumefaciens Cereal species Genome editing Genetic engineering Plant breeding 
基于微滴式数字PCR定量检测转基因玉米'CC-2'外源基因和内参基因在土壤中的存留被引量：2
《农业生物技术学报》2022年第5期1014-1022,共9页董姗姗 肖泽华 章嫡妮 刘燕 
国家自然科学基金(31901231);国家重点研发计划(2020YFC1806305-02)。
监测转基因作物外源基因在土壤中的持留水平及其动态变化,是评估转基因作物对土壤生态系统潜在风险的重要内容。本研究以耐除草剂转基因玉米(Zea mays)'CC-2'和非转基因对照玉米'郑58'为实验材料,采用微滴式数字PCR(droplet digital PCR...
关键词：转基因玉米'CC-2' 微滴式数字PCR(ddPCR) CP4-EPSPS(Agrobacterium tumefaciens strain CP45-enolpyruvyl shikimate-3-phosphate synthase) 基因水平转移 土壤生态系统 
Optimization of Agrobacterium tumefaciens-Mediated Genetic Transformation of Maize
《Phyton-International Journal of Experimental Botany》2022年第2期363-374,共12页Mengtong Liu Yangyang Zhou Tongyu Liu Jianyu Lu Jing Qu Shuyan Guan Yiyong Ma 
This work was supported by Jilin Provincial Natural Science Foundation Project[20190201168JC];“Thirteenth Five-Year”Science and Technology Project of Jilin Provincial Department of Education[JJKH20200341KJ];Science and Technology Project of Jilin Provincial Department of Education[JJKH20210351KJ,JJKH20210346KJ]Jilin Province Science and Technology Development Plan Project[20200402023NC];Jilin Province Key Technology R&D Project[20180201029NY];Jilin Province Support for the Science and Technology Development Program[20190802012ZG];College Student Innovation and Entrepreneurship Training Program Project[202010193087].Furthermore,the funding body have no role in designing of study,collection and interpretation of data or writing up the manuscript.
Immature embryos of inbred maize(Zea mays)lines(H8183,H8184,and H8185)were used for Agrobacterium infection.We used theβ-glucuronidase gene(GUS)as the target gene and the glufosinate resistance gene(bar)as the select...
关键词：MAIZE OPTIMIZATION GENETIC REGENERATION immature embryos Agrobacterium-mediated transformation 
Agrobacterium tumefaciens-mediated transformation of modern rose(Rosa hybrida)using leaf-derived embryogenic callus被引量：2
《Horticultural Plant Journal》2021年第4期359-366,共8页Guoqin Liu Yuan Yuan Hui Jiang Ying Bao Guogui Ning Liangjun Zhao Xiaofeng Zhou Hougao Zhou Junping Gao Nan Ma 
The authors thank Dr.Manzhu Bao(Huazhong Agricultural University,Wuhan,China),Dr.Hibrand-Saint Oyant L.(INRA,Agrocampus-Ouest,Universitéd’Angers,Beaucouzé,France)and Dr.Fabrice Foucher(INRA,78026 Versailles Cedex,France)for their excellent suggestions.We are also grateful to Dr.Wenxue Li and Dr.Hongqiu Wang(Chinese Academy of Agricultural Sci-ences,Beijing,China)for assistance with the experiments.This work was supported by grants from National Natural Science Foundation of China(Grant No.31522049);Construction of Beijing Science and Technology Innovation and Service Capacity in Top Subjects(Grant No.CEFF-PXM2019_014207_000032).
Rose(Rosa hybrida)is widely used for cut flowers and as garden plants.Stable and efficient transformation system is required for functional genomics of rose.Here,we established an efficient transformation method for r...
关键词：ROSE Rosa hybrida LEAF Somatic embryos Agrobacterium tumefaciens Genetic transformation 
Establishment of A Simple and Efficient Agrobacterium-mediated Genetic Transformation System to Chinese Cabbage(Brassica rapa L.ssp.pekinensis)被引量：7
《Horticultural Plant Journal》2021年第2期117-128,共12页Xiaonan Li Haiyan Li Yuzhu Zhao Peixuan Zong Zongxiang Zhan Zhongyun Piao 
the National key research and Development Program(Grant No.2017YFD0101802);the National Natural Science Foundation of China(Grant Nos.31772326 and 31701930);China Postdoctoral Science Foundation(Grant Nos.2016M601345 and 2019T120219).
Chinese cabbage,belonging to Brassica rapa species,is an important vegetable in Eastern Asia.It is well known that Chinese cabbage is quite recalcitrant to genetic transformation and the transgenic frequency is genera...
关键词：Chinese cabbage Agrobacterium tumefaciens genetic transformation 
Co-located quantitative trait loci mediate resistance to Agrobacterium tumefaciens, Phytophthora cinnamomi,and P.pini in Juglans microcarpa×J.regia hybrids
《Horticulture Research》2021年第1期1363-1373,共11页Ramesh K.Ramasamy Ming-Cheng Luo Charles A.Leslie Dianne Velasco Natalia Ott Ali McClean Abhaya M.Dandekar Mallikarjuna Aradhya Patrick J.Brown Gregory T.Browne Daniel A.Kluepfel Andreas Westphal Jan Dvorak 
This work was supported in part by USDA NIFA-SCRI grants no.59-5306-2-333 and 2018-51181-28437;the California Walnut Board,USDA ARS Project:2032-22000-016-00D,and NIFA-Hatch 1010599.
Soil-borne plant pathogens represent a serious threat that undermines commercial walnut(Juglans regia)production worldwide.Crown gall,caused by Agrobacterium tumefaciens,and Phytophthora root and crown rots,caused by ...
关键词：BREEDING regia soil 
Agrobacterium tumefaciens: A Bacterium Primed for Synthetic Biology被引量：2
《BioDesign Research》2020年第1期91-106,共16页Mitchell G.Thompson William M.Moore Niklas F.C.Hummel Allison N.Pearson Collin R.Barnum Henrik V.Scheller Patrick M.Shih 
This work conducted by the Joint BioEnergy Institute was supported by the US Department of Energy,Office of Science,Office of Biological and Environmental Research under contract no;DE-AC02-05CH11231 between Lawrence Berkeley National Laboratory and the US Department of Energy。
Agrobacterium tumefaciens is an important tool in plant biotechnology due to its natural ability to transfer DNA into the genomes of host plants.Genetic manipulations of A.tumefaciens have yielded considerable advance...
关键词：TRANSFORMATION enable rigorous 
Reliable and Efficient Agrobacterium tumefaciens-Mediated Genetic Transformation of Dianthus spiculifolius被引量：2
《Horticultural Plant Journal》2020年第3期199-204,共6页Jingang Wang Shiyue Liu Hongping Ma Ye Tao Shuang Feng Shufang Gong Jinzhu Zhang Aimin Zhou 
supported by grants from the National Natural Science Foundation of China(Grant Nos.31902052 and 31972450);the National Key Research and Development Program of China(Grant No.2016YFC0500300);the Natural Science Foundation of Heilongjiang Province of China(Grant No.C2018021);the‘Academic backbone’Project of Northeast Agricultural University of China(Grant No.18XG08).
Dianthus spiculifolius is a perennial herbaceous flower with strong environmental adaptability and is an important ornamental ground cover plant.In this study,seeds of D.spiculifolius were used as explants for callus ...
关键词：Agrobacterium tumefaciens Dianthus spiculifolius CALLUS Tissue culture Plant transformation 
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