BACILLUS_THURINGIENSIS

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Flexibility Analysis of Bacillus thuringiensis Cry1Aa
《Biomedical and Environmental Sciences》2015年第9期634-641,共8页ZHAO Xin Min XIA Li Qiu YANG Xiao Ping PENG Xiao Yun 
supported by grants from the National Natural Science Foundation of China(No.30670052);863 Program of China(No.2006AA02Z187)
Objective To investigate the flexibility and mobility of the Bacillus thuringiensis toxin Cry1 Aa. Methods The graph theory-based program Constraint Network Analysis and normal mode-based program NMsim were used to an...
关键词：Flexibility Cry1Aa Bacillus thuringiensis Constraint Network Analysis NMSim 
Comparison and optimization of the method for Cry1Ac protoxin preparation in HD73 strain被引量：12
《Journal of Integrative Agriculture》2015年第8期1598-1603,共6页ZHOU Zi-shan YANG Su-juan SHU Chang-long SONG Fu-ping ZHOU Xue-ping ZHANG Jie 
supported by the National Natural Science Foundation of China(31272115);the National High-Tech R&D Program(863 Program 2011AA10A203);the China Postdoctoral Science Foundation(2014M560487)
Bacillus thuringiensis is one of the most widely used bioinsecticides, and cry gene is the major insecticidal gene.Because Cry1 Ac protein shows strong toxicity against many lepidopteran species, it has been applied w...
关键词：Bacillus thuringiensis Cry1Ac protoxin crystal solubilization total yield TOXICITY 
Homology Modeling of Mosquitocidal Cry30Ca2 of Bacillus thuringiensis and Its Molecular Docking with N-acetylgalactosamine被引量：2
《Biomedical and Environmental Sciences》2012年第5期590-596,共7页ZHAO Xin Min ZHOU Pan Deng XIA Li Qui 
supported by grants from Hunan Provincial Natural Science Foundation of China(No.12JJ3021);the National Natural Science Foundation of China(No.30670052,30570050);863 Program of China(2006AA02Z187)
Abstract Objective To investigate the theoretical model of the three-dimensional structure of mosquitocida Cry3OCa2 and its molecular docking with N-acetylgalactosamine. Methods The theoretical model of Cry30Ca2 was t...
关键词：Cry3OCa2 Bacillus thuringiensis Homology modeling MOSQUITOES Molecular docking 
Transformation of Cabbage(Brassica oleracea L.var.capitata) with Bt cry1Ba3 Gene for Control of Diamondback Moth被引量：11
《Agricultural Sciences in China》2011年第11期1693-1700,共8页YI Deng-xia CUI Lei LIU Yu-mei ZHUANG Mu ZHANG Yang-yong FANG Zhi-yuan YANG Li-mei 
supported by the grants from the National High Technology Researchand Development Program of China (863 Program,2008AA10Z155);the National Natural Science Foundation of China (31071697);the earmarked fund for the Modern Agro-Industry Technology Research System,China (nycytx-35-gw01)
To obtain transgenic cabbage line with broad insect resistance, a new synthetic Bacillus thuringiensis crylBa3 gene was introduced into white cabbage via Agrobacterium tumefaciens-mediated transformation and 37 transf...
关键词：Bacillus thuringiensis crylBa3 CABBAGE diamondback moth resistance 
The role of β18-β19 loop structure in insecticidal activity of Cry1Ac toxin from Bacillus thuringiensis被引量：4
《Chinese Science Bulletin》2008年第20期3178-3184,共7页XIA LiQiu WANG FaXiang DING XueZhi ZHAO XinMin FU ZuJiao QUAN MeiFang YU ZiNiu 
the National High Technology Research and Development Program of China (Grant No. 2006AA02Z187, 2006AA10A212);National Natural Science Foundation of China (Grant No. 30670052);Ph.D. Programs Foundation of Ministry of Education of China (Grant No. 20060452006)
The β18-β19 loop in domain III of Cry1Ac toxin is unique among Bacillus thuringiensis Cry proteins. In this study, the role of the loop structure in insecticidal activity of Cry1Ac toxin was investigated. Alanine sc...
关键词：苏云金芽孢杆菌 CRY1AC 杀虫剂 杀虫活性 环结构 天冬酰胺酸 色氨酸 
有机溶剂对苏云金芽孢杆菌(Bacillus thuringiensis)几丁质酶的影响被引量：13
《应用与环境生物学报》2005年第1期71-73,共3页黄小红 陈清西 王君 沙莉 黄志鹏 关雄 
国家高技术研究发展计划 (2002AA245011 );福建省重大科技计划项目(2002N004)资助 ~~
　以苏云金芽孢杆菌(Bt)的发酵液为材料,经硫酸铵分级分离、DEAE-32离子交换柱层析分离,获得部分纯化的Bt几丁质酶(EC3. 2. 1. 14)制剂.研究了几种有机溶剂对Bt几丁质酶的影响.结果表明,甲醇、丙三醇、甲醛和戊二醛对几丁质酶有抑制作用...
关键词：苏云金芽孢杆菌 几丁质酶 有机溶剂 酶活力 
Susceptibility of Ostrinia furnacalis to Bacillus thuringiensis and Bt Corn Under Long-Term Laboratory Selection
《Agricultural Sciences in China》2005年第2期125-133,共9页WENLi-ping HEKang-lai WANGZhen-ying ZHOUDa-rong BAIShu-xiong 
the National 863 Program(2002AA212161); the National Natural Science Foundation of China(39970489).
The susceptibility of the Asian corn borer, Ostrinia furnacalis to Bacillus thuringiensis (Bt) formulation and Bt corn was evaluated using insect bioassays for 6 years. Four strains of O. furnacalis were developed by ...
关键词：Ostrinia furnacalis Bacillus thuringiensis Bt corn SUSCEPTIBILITY 
苏云金芽胞杆菌(Bacillus thuringiensis)几丁质酶的分离纯化及酶学性质被引量：13
《应用与环境生物学报》2004年第6期771-773,共3页黄小红 陈清西 王君 沙莉 黄志鹏 关雄 
国家高技术研究发展计划(2002AA245011);福建省重大科技计划项目(2002N004)的资助~~
以苏云金芽胞杆菌为材料 ,通过硫酸铵沉淀分级分离、DEAE - 32离子交换柱层析初步纯化 ,获得比活力为15 0 .5Umg-1的酶制剂 .研究酶催化胶体几丁质水解的反应时间和酶量对酶活力的关系 ,探讨酶催化胶体几丁质的最适条件 .温度和 pH对酶...
关键词：苏云金芽胞杆菌 几丁质酶 分离纯化 pH稳定性 温度稳定性 
苏云金芽孢杆菌Bacillus thuringiensis LSZ9408菌株发酵特性的研究被引量：4
《武夷科学》2004年第1期51-54,共4页周先治 刘波 黄素芳 孙新涛 
国家 8 6 3计划项目 (2 0 0 2 AA2 4 4 0 31- 2 )
本文研究了苏云金芽孢杆菌 LSZ94 0 8菌株的发酵特性 ,2 4 h菌体浓度达最大 ,2 8- 40 h菌体浓度稳定 ,4 0 h后菌体浓度下降 ,发酵液 p H值先降后升 ,最高达 p H7.8,2 0 h达到碳氮源利用高峰 ,2 8h后氮含量回升 ,糖含量基本不变。
关键词：苏云金芽孢杆菌 LSZ9408菌株 发酵特性 
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