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Analysis of Genomic Genetic Diversity of Cucurbit Aphidborne Yellows Virus
《Agricultural Biotechnology》2022年第4期17-21,25,共6页Wei CHEN Lina NIU Zhengxiu CUI Yongping LUO Juan HE Min CHAI Maimaiti YUSHANJIANG 
Supported by National Natural Science Foundation of China(31760511);Linfen Key R&D Program(2009);2020 National Key R&D Program-Technology Helps the Economy 2020 Key Project(2020-NK-ZL34);Fundamental Research Program of Shanxi Province(201901D211403)。
[Objectives]This study was conducted to investigate the molecular characteristics,genetic structure and evolutionary mechanism of cucurbit aphid-borne yellows virus(CABYV),which is of great significance for clarifying...
关键词：CABYV Genome cloning Genetic diversity 
Cloning and Bioinformatics Analysis of FaPHYC Gene in Tall Fescue (Festuca arundinacea)
《Agricultural Biotechnology》2019年第5期1-5,共5页Lu CAI Xiaoxia LIU Degang ZHAO Xiaoli WANG Xi CHEN 
Supported by project of High-level Innovative Talents Project in Guizhou Province([2018]5634);Youth Fund of Guizhou Academy of Agricultural Sciences(2018-80);Guizhou Province S&T Contract Basics([2019]1302)
The cDNA of PHYC gene of tall fescue ( Festuca arundinacea ) was cloned by RT-PCR,named FaPHYC.The sequence was analyzed by bioinformatic software,and the results showed that the full-length cDNA(3816 bp) was obtained...
关键词：FESTUCA arundinacea FaPHYC GENE GENE CLONING BIOINFORMATICS analysis 
Bioinformatics and Expression Analysis of CaERF Gene in Capsicum
《Agricultural Biotechnology》2019年第4期26-28,共3页Jiaqi GENG Jieyu YANG Peiqi HU Ziqi ZHAO Kunpeng LI Jingying WANG Chunyu ZHANG Qingxun GUO 
Supported by College Students’Innovation Fund of Jilin University(2017A82372)
AP2/ERF transcription factor is a kind of plant-specific transcription factor,which is widely involved in the whole process of plant growth and development,and has important regulatory effects in plant secondary metab...
关键词：CAPSAICIN CaERF GENE GENE CLONING mRNA expression 
Cloning of Ds MAPK Gene and Construction of Antisense Expression Vector
《Agricultural Biotechnology》2018年第4期8-11,24,共5页Jinrong YUE Yuting CONG Xiangnan GAO Zhenyu XING Wenjing YUE Xiaojie CHAI 
Supported by National Natural Science Foundation of China(31472260,30972240)
In order to investigate the role of MAPK gene in adaptation of Dunaliella salina to hypersaline environment, the Ds MAPK gene of D. salina was amplified by PCR. After inverted insertion of the open reading flame ofDs ...
关键词：Dunaliella salina Ds MAPK Antisense expression vector Semi-quantitative RT-PCR 
Cloning and Diversity Analysis of TaHKT2;2 in Hexaploid Wheat
《Agricultural Biotechnology》2018年第4期16-20,54,共6页Mengjun LI Yaqing LI Shichang ZHANG Nan ZHANG Zhanliang SHI 
Supported by National Key Research and Development Program of China(2016YFD0101802);Modern Agricultural Science and Technology Innovation Engineering Project of Hebei Province(2017038997);National Natural Science Foundation of Hebei Province(C2014106075)
In order to study the roles of members of HKT gene fanfily in wheat, TaHKT2 ; 2 was isolated by using homologous cloning strategy and screening genomic BAC library. TaHKT2; 2 genes were mapped on chromosomes 7A, 7B an...
关键词：Hexaploid wheat HKT gene family TaHKT2 2 BAC library DIVERSITY 
Molecular Cloning and Sequence Analysis of C4H Gene of Mangifera indica L.
《Agricultural Biotechnology》2017年第5期6-10,共5页Kuanliang LIU Zhichang ZHAO Aiping GAO Yeyuan CHEN Jianfeng HUANG Zhiguo DANG Ruixiong LUO 
Supported by National Natural Science Foundation of China(31471850);the Fund for the Protection of Tropical Crops Genetic Resources(15RZZY-07);"948"Program of the Ministry of Agriculture of China(2011-G13);the Startup Fund for the Reform of Nonprofit Scientific Research Institutions(CATAS PZS-201225,CATAS-TCGRI 1630032013003)
Cinnamate-4-hydroxylase( C4H) is a key enzyme in phenylpropanoid pathway in plants. Its activity and abundance directly affect the biosynthesis of flavonoids and aromatic compounds. In this study,degenerate primers we...
关键词：Mangifera indica L. C4H gene CLONING PROTEIN Bioinformatics analysis 
Cloning of Small Heat-shock Protein (sHSP) Gene from Sugarcaneand Analysis of Its Expression under Drought Stress被引量：2
《Agricultural Biotechnology》2017年第2期6-10,14,共6页Panxia LIANG Jiangtao ZHOU Xing HUANG Yangrui LI 
Supported by National High Technology Research and Development Program of China(863program)(2013AA102604);International Cooperation Project of Ministry of Science and Technology(2013DFA31600);Guangxi Natural Science Foundation(2014GXNSFBA118139);Special Fund for Basal Scientific Research of Guangxi Academy of Agricultural Sciences(GN2014YD13)
The present experiment was conducted to clone small heat-shock protein （sHSP） in sugarcane to explore mechanism of stress resistance in sugarcane. sHSP was amplified using RT-PCR from sugarcane leaves, the character...
关键词：SUGARCANE DROUGHT Silicon HSP 
Cloning and Analysis of TasA Gene in Bacillus subtilis HAS
《Agricultural Biotechnology》2017年第2期48-50,54,共4页Guoruo XIONG Gengfeng ZHAO Wenwei CAI Suran WU Benpeng YANG Shuzhen ZHANG 
Supported by National Natural Science Foundation of China(31471555);Natural Science Foundation of Hainan Province(314120);Earmarked Fund for Modern Agro-industry Technology Research System of China(nycytx-24)
In order to clarify the mechanism for the inhibitory action of the Bacillus subtilis strain HAS on Sporisorium scitamineum （ Syd. ）, which can cause sugarcane smut, the full-length TasA gene which encodes a protein ...
关键词：Bacillus subtilis HAS mad a glutamic acid was replaced with an asparagine. Sporisorium scitamineum （ Syd. ） Antimicrobial protein TasA 
Cloning and Functional Analysis of Xylem Specific Promoter Deletion Fragment
《Agricultural Biotechnology》2016年第6期1-3,9,共4页Yajing LUO Liang WANG Kejiu DU Shuang ZHANG 
Supported by National Science Foundation of China(31000305);Fund from Education Department of Hebei Province(QN2015182);Innovation Fund for Forestry Discipline of Hebei Agricultural University(LXXK2014-1);Fund for Overseas Research and Study by Young and Middle-aged Backbone Teachers in Hebei Agriculture University(JWYX2015)
Xylem-specific promoter could regulate efficient expression of foreign genes in xylem. Deletion analysis method was applied to obtain 5＇ deletion promoter fragments MU2 andMU4 with different distances from transcript...
关键词：PROMOTER Deletion analysis Vector construction GUS activity analysis 
16S rRNA Cloning and Identification of a Strain Isolated from Intestinal Tract of Rex Rabbit被引量：1
《Agricultural Biotechnology》2016年第5期37-41,共5页Guangjun GUO Yumao WANG Sufang LYU Jing HAN Xinming WU Hanjie ZHANG Qiqi ZOU Qiang FU Zhiqiang SHEN 
Supported by Modern Agricultural Technology System Construction Project of Shandong Province(SDAIT-21-15);Shen Zhiqiang Innovation Team Program of Animal Science and Veterinary Service of Shandong Province(LFFW[2014]01041)
This study was conducted to identify bacterial strain HeNan-001 isolated from intestinal tract of healthy rex rabbit. The strain was identified by gram staining, and OD600 values at different culture time were measure...
关键词：Diplostreptococcus Sequences analysis Strain identification 16S rRNA 
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