MAMMALIAN_CELLS

作品数：89被引量：390H指数：7
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Lipid profiling of mammalian cells with in situ matrix-assisted laser desorption ionization-mass spectrometry被引量：1
《Science China Chemistry》2014年第3期442-446,1-3,共5页ZHANG YanDong LI HaiFang MA Yuan LIN JinMing 
supported by the National Natural Science Foundation of China(21227006,21275088)
The analysis of cellular lipids and phospholipids has been of continuously increasing research interest due to the importance of these molecules in psychological process.In this work,a mass spectrometry-based method f...
关键词：lipid profiling MALDI-MS linear discriminative analysis 
An integrated microfluidic device for long-term culture of isolated single mammalian cells被引量：2
《Science China Chemistry》2012年第4期502-507,共6页ZHENG ChunHong CHEN Gui’E PANG YuHong HUANG YanYi 
supported by the National Natural Science Foundation of China (20733001, 20890020, 90913011, 20905004);the Ministry of Science and Technology of China (2011CB809106);the Ministry of Education of China;the Fok Ying Tung Education Foundation
We developed an integrated microfluidic chip for long-term culture of isolated single cells. This polydimethylsiloxane (PDMS) based device could accurately seed each single cell into different culture chambers, and is...
关键词：MICROFLUIDICS single cell culture conditioned medium 
AN HBV LARGE SURFACE ANTIGEN PROTEIN WHICH CAN BE SECRETED FROM MAMMALIAN CELLS
《Science China Chemistry》1992年第4期455-462,共8页俞贤明 汪垣 李载平 
This research was supported in part by U. S. Public Health Service Grants CA-70175 and CA-2243 to J. Mertz from the U. S. National Institute of Health.
The N-terminal 54 base pairs (encoding amino acid residues 2—19) within the preS1 region of the human hepatitis B virus surface antigen gene were deleted by site-directed mutagenesis. Unlike the wild type large surfa...
关键词：HEPATITIS B virus LARGE SURFACE ANTIGEN protein secretability retention sequence. 
TRANSFER OF FOREIGN GENES BY ELECTROPORATION AND THEIR EXPRESSION IN MAMMALIAN CELLS
《Science China Chemistry》1989年第5期543-550,共8页杨建涛 王秀琴 吴旻 
Expression of foreign genes transferred into mammalian cells by electroporation has been studied. The pX1TK gene, pSV2Neo gene and pUCEJ oncogene have been introduced into MLTK-cells and NIH/3T3 cells, respectively. S...
关键词：GENE TRANSFER GENE EXPRESSION ONCOGENE electroporation. 
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