METABOLIC_ENGINEERING

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Creation of purple leaf peanut germplasm through metabolic engineering of the betalain biosynthesis pathway
《Journal of Integrative Agriculture》2025年第4期1606-1609,共4页Dongxin Huai Jie Wu Xiaomeng Xue Hao Liu Nian Liu Li Huang Liying Yan Yuning Chen Xin Wang Qianqian Wang Yanping Kang Zhihui Wang Yanbin Hong Huifang Jiang Boshou Liao Yong Lei 
supported by the Guangdong Provincial Key Research and Development Program-Modern Seed Industry,China(2022B0202060004);the Knowledge Innovation Program of Wuhan-Basic Research,China(2022020801010291);the Project of the Development for High-quality Seed Industry of Hubei Province,China(HBZY2023B003);the Innovation Program of the Chinese Academy of Agricultural Sciences(2023-2060299-089-031)。
With the progress of urbanization,rural tourism has emerged as a popular leisure activity in China.The crop field with pattern art has been gaining popularity over the years,using strains with colorful leaves to creat...
关键词：ornamental agriculture rural tourism metabolic engineering betalain biosynthesis urbanization crop field pattern art colorful leaves strains colorful leaves 
Harnessing metabolomics for enhanced crop drought tolerance
《The Crop Journal》2025年第2期311-327,共17页Ali Raza Muhammad Anas Savita Bhardwaj Rakeeb Ahmad Mir Sidra Charagh Minhas Elahi Xinyue Zhang Reyazul Rouf Mir Wolfram Weckwerth Alisdair R.Fernie Kadambot H.M.Siddique Zhangli Hu Rajeev K.Varshney 
supported by Chinese National Key R&DProject for Synthetic Biology(2018YFA0902500);National Natural Science Foundation of China(32273118);The Guangdong Key R&D Project(2022B1111070005);Shenzhen Special Fund for Sustainable Development(KCXFZ20211020164013021);Shenzhen University 2035 Program for Excellent Research(2022B010);supported by a startup grant from the Food Futures Institute of Murdoch University,Australia.
Global crop productivity faces a significant threat from climate change-induced drought stress(DS),which is vital for sustainable agriculture and global food security.Uncovering DS adaptation and tolerance mechanisms ...
关键词：Amino acids Climate change Food security mQTL and mGWAS Metabolic engineering Single-cell metabolomics Water scarcity 
Combinatorial metabolic engineering of Escherichia coli for de novo production of structurally defined and homogeneous Amino oligosaccharides
《Synthetic and Systems Biotechnology》2024年第4期713-722,共10页Jinqi Shi Chen Deng Chunyue Zhang Shu Quan Liqiang Fan Liming Zhao 
supported by the Young Scientists Fund of the National Natural Science Foundation of China(32301214);Shanghai Post-doctoral Excellence Program(2022148);the 111 Project(B18022).
Amino oligosaccharides(AOs)possess various biological activities and are valuable in the pharmaceutical,food industries,and agriculture.However,the industrial manufacturing of AOs has not been realized yet,despite rep...
关键词：Amino oligosaccharides Chitin oligosaccharides Metabolic engineering Fed-batch cultivation bioproduction 
Engineering Corynebacterium glutamicum for the efficient production of 3-hydroxypropionic acid from glucose via theβ-alanine pathway
《Synthetic and Systems Biotechnology》2024年第4期752-758,共7页Xiaodi Wang Junyuan Hou Jieyao Cui Zhiwen Wang Tao Chen 
supported by the National Key Research and Development Program of China(2021YFC2100700);the National Natural Science Foundation of China(NSFC-21776208).
3-Hydroxypropionic Acid(3-HP)is recognized as a high value-added chemical with a broad range of applications.Among the various biosynthetic pathways for 3-HP production,theβ-alanine pathway is particularly noteworthy...
关键词：3-Hydroxypropionic acid β-Alanine pathway Corynebacterium glutamicum Metabolic engineering 
Screening and characterization of integration sites based on CRISPR-Cpf1 in Pichia pastoris
《Synthetic and Systems Biotechnology》2024年第4期759-765,共7页Shupeng Ruan Yuxin Yang Xinying Zhang Guanjuan Luo Ying Lin Shuli Liang 
supported by the National Key Research and Development Program(2021YFC2104000);the National Natural Science Foundation of China(32272276);the Fundamental Research Funds for the Central Universities.
Pichia pastoris,a methylotrophic yeast,can utilize methanol as a carbon source and energy source to synthesize high-value chemicals,and is an ideal host for biomanufacturing.Constructing the P.pastoris cell factory is...
关键词：P.PASTORIS CRISPR-Cpf1 Integration sites Metabolic engineering 
Stepwise increase of fidaxomicin in an engineered heterologous host Streptomyces albus through multi-level metabolic engineering
《Synthetic and Systems Biotechnology》2024年第4期766-774,共9页Huang Xie Yi-Ting Su Qing-Ting Bu Yue-Ping Li Qing-Wei Zhao Yi-Ling Du Yong-Quan Li 
supported by the National Key R&D Program of China(grant number 2019YFA09005400);the Zhejiang Provincial Natural Science Foundation of China(grant number LQ21C010002).
The anti-Clostridium difficile infection(CDI)drug fidaxomicin is a natural polyketide metabolite mainly produced by Micromonosporaceae,such as Actinoplanes deccanensis,Dactylosporangium aurantiacum,and Micromonospora ...
关键词：Fidaxomicin Heterologous expression Streptomyces albus Genome-reduced chassis Metabolic engineering 
Screening of ent-copalyl diphosphate synthase and metabolic engineering to achieve de novo biosynthesis of ent-copalol in Saccharomyces cerevisiae
《Synthetic and Systems Biotechnology》2024年第4期784-792,共9页Shan Li Shuangshuang Luo Xinran Yin Xingying Zhao Xuyang Wang Song Gao Sha Xu Jian Lu Jingwen Zhou 
supported by the National Natural Science Foundation of China(22278188);the National Natural Science Foundation of China(22208123);the Starry Night Science Fund of Zhejiang University Shanghai Institute for Advanced Study(Grant No.SN-ZJU-SIAS-0013);the National First-class Discipline Program of Light Industry Technology and Engineering(QGJC20230102).
The diterpene ent-copalol is an important precursor to the synthesis of andrographolide and is found only in green chiretta(Andrographis paniculata).De novo biosynthesis of ent-copalol has not been reported,because th...
关键词：Andrographis paniculata Ent-copalyl diphosphate synthase ent-CPP Diterpene synthase ACETYL-COA Quantification 
Cyanamide-inducible expression of homing nuclease ^(I−)SceI for selectable marker removal and promoter characterisation in Saccharomyces cerevisiae
《Synthetic and Systems Biotechnology》2024年第4期820-827,共8页Liam McDonnell Samuel Evans Zeyu Lu Mitch Suchoronczak Jonah Leighton Eugene Ordeniza Blake Ritchie Nik Valado Niamh Walsh James Antoney Chengqiang Wang Carlos Horacio Luna-Flores Colin Scott Robert Speight Claudia E.Vickers Bingyin Peng 
supported by the Australian Government through the Australian Research Council Centres of Excellence funding scheme(project CE200100029).
In synthetic biology,microbial chassis including yeast Saccharomyces cerevisiae are iteratively engineered with increasing complexity and scale.Wet-lab genetic engineering tools are developed and optimised to facilita...
关键词：Metabolic engineering Genetic circuit Genome engineering Synthetic biology Yeast engineering Promoter engineering 
Heterologous biosynthesis of saponin adjuvants from Quillaja saponaria:A symbolic achievement in metabolic engineering
《Acta Pharmaceutica Sinica B》2024年第12期5518-5520,共3页Weiqiang Chen Zhichao Xu Wei Sun 
Heterologous production of pharmaceutically important plant nat-ural products(PNPs)in heterologous hosts is an attractive strategy for the sustainable use of the resources bestowed by nature.Over the past two decades,...
关键词：QS-21 QS-7 Plant natural products(PNPs) Heterologous biosynthesis 
Editing function of type Ⅱ thioesterases in the biosynthesis of fungal polyketides
《Chinese Chemical Letters》2024年第10期325-328,共4页Fenglin Jiang Anan Liu Qian Wei Youcai Hu 
supported financially by the National Natural Science Foundation of China(Nos.82225042,T2192973);the CAMS Innovation Fund for Medical Sciences(No.CIFMS 2021-I2M1-029)。
Polyketide synthases(PKSs)are megasynthases with multiple autonomously folding domains,which operate cooperatively in the PKS assemblies to synthesize specific polyketide scaffolds.Any nonreactive intermediates tether...
关键词：Editing enzyme Type II thioesterase BIOSYNTHESIS Polyketide synthase Metabolic engineering 
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