Acknowledgments This work was supported by the National Basic Research Program (2013CB910200 and 2014CB910700), the National Natural Science Foundation of China (31222017), and the Ministry of Education of PRC (NCET-11-0874). We thank Xuefeng Zhu (University of Gothenburg, Sweden) for providing the TP 161 strain for expression of S. pombe Mediator.
The Mediator complex plays an essential role in the regulation of eukaryotic transcription. The Saccharomyces cerevisiae core Mediator comprises 21 subunits, which are organized into Head, Middle and Tail modules. Pre...