荧光假单胞菌AR4产2-酮基-D-葡萄糖酸发酵条件的研究  

Studies on the Fermentation Conditions of Pseudomonas fluorescens AR4 Producing 2-Keto-D-gluconic Acid

作　　者：孙文敬[1,2] 杨庆文[3] 黄惠英 郭金权[3] 赵峰梅[3] 刘敬泽[1] 

机构地区：[1]河北师范大学生命科学学院 [2]山西省生物研究所,山西太原030006 [3]山西省生物研究所 [4]郑州市生物化工厂

出　　处：《食品科学》2004年第10期46-49,共4页Food Science

摘　　要：以淀粉水解糖为碳源,研究了不同氮源、碳氮比、金属离子、接种量以及环境因子(培养基起始pH值、通气量、温度)对抗噬菌体菌株荧光假单胞菌(Pseudomonas fluorescens)AR4产2-酮基-D-葡萄糖酸发酵的影响。结果表明,氮源、碳氮比、培养基的起始pH值、通气量及温度对该菌的产酸均有显著影响。在适宜培养条件下,该菌的摇瓶产酸及发酵转化率分别可达13.5%和90%左右。Using saccharide from corn starch hydrolysate as a sole carbon source, the factors (nitrogen sources, carbon-nitrogenratio, metal ions, the volume of inoculation, initial pH value of the medium, aeration rate and temperature) affecting 2-keto-D-gluconic acid fermentation by bacteriophage-resistant mutant Pseudomonas fluorescens AR4 were investigated. The resultsshowed that the production of 2-keto-D-glluconic acid was significantly affected by nitrogen sources, carbon-nitrogen ratio,initial pH value of the medium, aeration rate and temperature. Under the suitable fermentation conditions, the acid yield and theconversion rate was about 13.5% and 90% respectively.

关 键 词：荧光假单胞菌AR4 2-酮基-D-葡萄糖酸 发酵 抗噬菌体菌株 

分 类 号：TS201.3[轻工技术与工程—食品科学]