紫外诱变筛选低高级醇和双乙酰含量的啤酒酵母  

Screening of low higher-alcohols and diacetyl producing Saccharomyces cerevisiae strain with ultraviolet mutagenesis

作　　者：汪志君[1] 高庆[1] 方维明[1] 姜涌明[1] 颜荣林 周红 

机构地区：[1]扬州大学食品科学与工程学院,江苏扬州225001 [2]青岛啤酒(扬州)有限公司,江苏扬州225001

出　　处：《中国酿造》2005年第1期13-17,共5页China Brewing

摘　　要：根据酵母相关代谢途径,将降低高级醇和双乙酰含量为目的进行育种。采用紫外诱变方法对啤酒酵母进行处理,用乳酸平板、麦芽汁-碳酸钙平板、TTC上层平板进行显色分离,最终得到1株高级醇产量降低约30%、双乙酰含量亦较低的新菌株。Saccharomyces cerevisiae was selected to get low higher-alcohols and diacetyl producing strain according to its correlative metabolic pathway. S. cerevisiae mutagenized by ultraviolet was isolated with lactic acid plate, wort-CaCO3 plate, and TTC upper layer plate. A new strain, which higher alcohols production was 30% lower and diacetyl content was also lower than that of original strain, was obtained finally.

关 键 词：啤酒酵母 紫外诱变 高级醇 双乙酰 

分 类 号：Q813.5[生物学—生物工程]