福建老酒中血管紧张素转换酶抑制物质的分离鉴定  

Isolation and Identification of Substances with Angiotensin Converting Enzyme Inhibitory Activity in Fujiang Rice Wine

作　　者：张蓉真[1] 刘树滔[1] 陈儒明[1] 李建才[1] 李珑[1] 饶平凡[1] 

机构地区：[1]福州大学生物工程研究所

出　　处：《福州大学学报（自然科学版）》1996年第6期114-118,共5页Journal of Fuzhou University(Natural Science Edition)

摘　　要：福建老酒的样品经在ＴｏｙｏｐｅａｒｌＨＷ４０５凝胶过滤色谱柱分离成包括蛋白质、肽、乙醇和糖类等５个主要组分，其中含肽类物质的第二组分显示出较强的ＡＣＥ抑制活性，该组分经ＳｕｐｅｒｄｅｘＰｅｐｔｉｄｅＨＲ１０／３０进一步分离纯化，获得３个组分．其中的第二及第三组分具有ＡＣＥ抑制活性，其ＩＣ（５０）分别为２．８及８．１μｇＰｒｏｔｅｉｎ／ｍＬ，而分子量则为１１００和４５０．Fujian Rice Wine was fractionated into 5 fractions which contained proteins,peptides,ethonal and sugar by gel chromatography on Toyopearl HW-40S column.The second fraction of peptides exhibited strong angiotensin converting enzyme(ACE)inhibitory activity.It was further fractionated into 3 fractions by gel chromatography on Super Peptide HR10/30 column.The second and the third fractions obtained exhibited ACE inhibitory activity.Their IC50s were 2.8μg protein/mL and 8.1μg protein/mL, corresponding to molecular weights 1 100 and 450,and amino acid compositions of A2C2LE2TC and GIEV,respectively.

关 键 词：福建老酒 肽类 ACE抑制活性 黄酒 ACE 

分 类 号：TS262.4[轻工技术与工程—发酵工程] TS201.25[轻工技术与工程—食品科学与工程]