粗壮脉纹孢菌液体发酵产纤维素酶的条件优化  

The optimal liquid fermentation conditions of cellulase production by neurospora crassa

作　　者：吴丹[1] 邓泽元[2] 范亚苇[2] 余玮[2] 

机构地区：[1]南昌大学食品科学教育部重点实验室,江西南昌330047 [2]南昌大学食品科学与工程系,江西南昌330047

出　　处：《食品与机械》2007年第3期25-27,共3页Food and Machinery

基　　金：教育部"长江学者和创新团队发展计划资助"(项目编号:IRT0540);江西省教育厅科研项目(项目编号:200626)

摘　　要：研究了粗壮脉纹孢菌发酵稻草粉生产纤维素酶的影响因素及稻草秸杆浓度、培养温度、初始pH值对内切葡聚糖酶活、滤纸酶活和菌体生长的影响。初始pH4.8,培养温度30℃,稻草浓度5%,发酵时间为6d时,内切酶酶活和滤纸酶活均最高。而菌体生长的最适条件为:初始pH5.5,前3d用25℃培养,后期改用30℃培养,稻草浓度为2%。The production of the cellulase complex by the fungus Neurospora crassa on milled and sieved rice straw was studied. The effects of straw concentration, temperature, and initial pH on the production of endoglucanase and filter paper activities, as well as mycelia protein, were observed. With an initial pH value of 4.8, a straw concentration of 5% and a temperature of 30 ℃ for 6 days yielded the higher activities for the two enzymes. The optimal condition of Neurospora erassa growing in submerged fermentation were as follows： with an initial pH value of 5.5, a straw eoncentration of 2% and the changed temperature of a starting temperature of 25 ℃ and a final temperature of 30 ℃.

关 键 词：纤维素酶 粗壮脉纹孢菌 稻草 发酵 

分 类 号：TQ925[轻工技术与工程—发酵工程]