酶法制备低聚木糖的研究  

Study on preparation of xylooligosaccharides with enzyme

作　　者：韩玉洁 徐冬[1] 徐忠[1] 

机构地区：[1]哈尔滨商业大学食品学院,哈尔滨150076

出　　处：《哈尔滨商业大学学报（自然科学版）》2007年第3期267-272,共6页Journal of Harbin University of Commerce：Natural Sciences Edition

基　　金：黑龙江省自然科学基金项目:B0304;黑龙江省教育厅科学技术研究项目

摘　　要：利用木聚糖酶酶解小麦麸皮制备低聚木糖,为麸皮的深加工开辟了一条新途径.根据实验确定了木聚糖酶水解麸皮木聚糖的工艺条件,即酶浓度为1200 IU/g底物,木聚糖终质量分数为12%,水解温度为40℃,水解时间为6 h,总糖得率为57.12%,低聚木糖的得率为22.52%.对酶解液进行定性定量分析,结果表明,其主要成分为木二糖和木三糖.In this paper, wheat bran is treated by xylanase to produce xylooligosaccharides. It opened a new way for the depth process of wheat bran. The processing parameters of xylan hydrolysis by xylanase are optimized as the substrate concentration 12%, xylanase dosage 1200 IU per gram substrate on 40 ℃ for 6 h. Under this condition, total sugar is 57.12%, the yield of xylooligosaccharides is 22.52%. The results of qualitative and quantitative analysis showed that the main componients of the enzymatic hydrolysates are xylobiose and xylotriose.

关 键 词：小麦麸皮 低聚木糖 木聚糖酶 酶法水解 酶解液 

分 类 号：TS201.2[轻工技术与工程—食品科学]