莲藕渣中多糖的提取及性质初步研究  

Extractation and Characteristics of Polysaccharides from Lotus Root Pomace

作　　者：严浪[1] 张树明[1] 张凡华[1] 石宝霞[1] 李淑燕[1] 李全宏[1] 

机构地区：[1]中国农业大学食品科学与营养工程学院,北京100083

出　　处：《食品科学》2007年第12期226-230,共5页Food Science

摘　　要：本实验综合利用提取淀粉后的莲藕渣提取水溶性多糖和不溶性膳食纤维,并对水溶性多糖的结构及不溶性膳食纤维的特性进行了初步研究。通过水提醇沉、Sevag法去蛋白得到粗多糖NPh,得率为2.50%。离子交换柱层析法分离纯化出莲藕多糖NPh1和NPh2,凝胶过滤柱色谱及高效液相色谱仪检测多糖纯度,证明NPh2为纯品,其相对分子量>2000kD,是一种酸性杂多糖,单糖组成为半乳糖,阿拉伯糖、鼠李糖、葡萄糖、岩藻糖和木糖,摩尔比依次为:26.74;16.17:5.69:5.49:2.31:1。利用碱化学法提取不溶性膳食纤维NDFh,得率为26.97%。用2.0%H2O2进行了脱色。NDFh的持水性和溶胀性分别为7.58g/g和9.0ml/g。For comprehensive use of the pomace of lotus roots, water-soluble polysaccharides and insoluble dietary fibre were extracted and their characteristics were studied as well. Polysaccharides were obtained by hot-water extraction, ethanol precipitation, sevag method removing protein and then purificated by DEAE Sepharose Fast-flow, and NPhl and NPh2 were fractioned. HPLC showed NPh2 was a pure acid polysaccharide with relative molecule weight more than 2000 kD that mainly composed of galactose, arabinose, rhamnose, glucose, fucose and xylose with molar ratio of 26.74： 16.17： 5.69： 5.49：2.31：1. Dietary fibre being gained by alkaline hydrolyzation and decolored by H202, the yield was 26.97% and its water-holding capability and swelling power were 7.58 g/g and 9.0 ml/g, respectively. Results showed that lotus roots pomace can be a good source of water-soluble polysaccharides and insoluble dietary fibre.

关 键 词：莲藕 渣 多糖 膳食纤维 

分 类 号：S667[农业科学—果树学]