正交试验优化添加天麻的黑木耳多糖发酵培养基  

Optimization of Auricularia Auricula Extro-Polysaccharide Fermentation Medium by Orthogonal Experiment Design

作　　者：张大为[1] 赵亮[2] 吴天祥[1] 

机构地区：[1]贵州大学化学工程学院,贵州贵阳550003 [2]贵州大学生命科学学院,贵州贵阳550025

出　　处：《贵州工业大学学报（自然科学版）》2007年第6期40-43,共4页Journal of Guizhou University of Technology(Natural Science Edition)

基　　金：贵州省高层次人才特助基金项目(GZ2006001)

摘　　要：研究天麻对黑木耳深层发酵多糖的影响。通过单因素实验确定了碳源、氮源、天麻和无机盐的添加水平。用正交试验优化添加天麻的黑木耳发酵培养基。优化后培养基组成为:葡萄糖40 g/L、酵母膏13 g/L、天麻10 g/L、KH2PO45 g/L、MgSO42.5g/L.Rhizoma Gastrodiae was involved in this paper to study the extro - polysaccharide fermentation of Auricularia Auricula by submerged culture. The levels of carbon rescores, nitrogen re.scores, salt and Rhizoma Gastrodiae was determined at first. And then the fermentation medium was optimized by orthogonal experiment design as well. The results show that the best medium composition was glucose 40g/L, yeast extract 13g/L, Rhizoma Gastrodiae 10g/L, KH2PO4 and MgSO4 5g/L and 2.5g/L respectively.

关 键 词：天麻 黑木耳 胞外多糖 深层发酵 

分 类 号：TQ920.61[轻工技术与工程—发酵工程]