复合诱变选育酸性蛋白酶高产菌株  

Screening of high acid protease producing strains with compound mutagenesis

作　　者：卢燕云[1] 林建国[1,2] 李明[1] 邱雁临[1,2] 胡瑛[1,2] 王常高[1,2] 蔡俊[1,2] 

机构地区：[1]湖北工业大学生物工程学院,湖北武汉430068 [2]湖北省工业微生物重点实验室,湖北武汉430068

出　　处：《中国酿造》2009年第1期49-51,共3页China Brewing

基　　金：2007年度湖北工业大学高层次人才启动基金

摘　　要：以米曲霉M-2为出发菌株,通过紫外线-氯化锂和硫酸二乙脂(DES)复合诱变,筛选酸性蛋白酶高产突变株,并进行固态发酵筛选试验。从大量突变株中进行筛选,获得1株遗传性稳定的酸性蛋白酶高产突变株D-7,其酶活力由出发菌的262.7U/g提高至602.5U/g,提高了129.3%。Using Aspergillus oryzae M-2 as original strain, the high acid protease producing strains were screened after compound mutagenesis of UV-LiCl and diethy sulfate （DES）. Furthermore, a mutant named D-7, which produced acid protease at high level with genetic stability, was selected by solid fermentation test. The protease activity of D-7 was 602.5 U/g, which was increased 129.3% compared with 262.7 U/g, the protease activity of M-2.

关 键 词：酸性蛋白酶 米曲霉 复合诱变 

分 类 号：Q813.5[生物学—生物工程]